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  1. Link

    products/secondary-antibodies/goat-chicken-igy-hl-alexa-fluor-488-ab150169.pdf

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Secondary antibodies anti-Chicken IgY Fluorophore Alexa Fluor® 488
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Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (121)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
  • Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)

Key features and details

  • Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 FITC Gold 10nm Gold 25nm HRP HRP PE Texas Red ® Unconjugated Unconjugated

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Overview

  • Product name

    Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)
    See all IgY secondary antibodies
  • Host species

    Goat
  • Target species

    Chicken
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Fluorophore
    • Alexa Fluor® 488

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (1)
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P (1)
Use at an assay dependent concentration.
Flow Cyt
1/2000.
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/2000.

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)

    ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab89984, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab150169 Alexa Fluor® 488 goat anti-chicken IgY (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
    Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (121)

Publishing research using ab150169? Please let us know so that we can cite the reference in this datasheet.

ab150169 has been referenced in 121 publications.

  • Quiñones M  et al. Sirt3 in POMC neurons controls energy balance in a sex- and diet-dependent manner. Redox Biol 41:101945 (2021). PubMed: 33744652
  • Yanai S  et al. Gene expression and functional abnormalities in XX/Sry Leydig cells. Sci Rep 11:719 (2021). PubMed: 33436964
  • Gessara I  et al. Highly Efficient Genome Modification of Cultured Primordial Germ Cells with Lentiviral Vectors to Generate Transgenic Songbirds. Stem Cell Reports 16:784-796 (2021). PubMed: 33740464
  • Arguello JR  et al. Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants. Elife 10:N/A (2021). PubMed: 33666172
  • Zhao T  et al. Loss-of-function of p53 isoform ?113p53 accelerates brain aging in zebrafish. Cell Death Dis 12:151 (2021). PubMed: 33542214
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

primary: chicken anti GFAP (ab4674) secondary: ab150169

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
1:500 dilution in Blocking solution (10% NGS, 0,3% TritonX100)

Incubation time: 1h

Good staining results!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 22 2020

Goat anti Chicken AF488

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Dilution secondary AB: 1:250
Dilution Buffer: Normal Serum
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 10 2020

ICC of Mouse brain organotypic slices

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Secondary anti-body worked well and gave strong clear staining in my samples
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Kirsty Ireland

Verified customer

Submitted Mar 18 2014

Question

I am looking for a secondary antibody for Anti-GFP antibody (ab13970) (made in chicken).

Would this be an appropriate secondary antibody?

Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)

(I’m wondering if the anti-chicken IgG is better)

Read More

Abcam community

Verified customer

Asked on Dec 10 2014

Answer



I am happy to confirm that ab150169 (Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)) is a suitable secondary antibody for ab13970 (Anti-GFP antibody (ab13970)).

I would like to note that chicken IgY and chicken IgG are the same kind of immunoglobulin.

The nomenclature sometimes is not very clear as IgY is often mislabeled as Immunoglobulin G (IgG) in older literature, and sometimes in commercial product catalogs, due to its functional similarity to mammalian IgG

Chicken IgY is the major circulating antibody found in chickens and is the avian counterpart to mammalian IgG’s. IgYs are equivalent to IgGs in most applications, including Western Blotting, ELISAs, immunohistochemistry, immunocytochemistry.

IgYs have the same structure as a mammalian IgG, with 2 heavy (“nu” chains, ˜67-70 kDa) and 2 light chains (22-30 kDa)

Unlike the gamma-chains of IgG, however, the heavy chains of IgYs lack Fc domains, which means that IgYs do not fix complement nor bind protein A or protein G.
Chickens also produce an alternatively-spliced form of the heavy chain in which the Cnu3 and Cnu4 domains are deleted. In some individual animals, this F(ab)2-like heavy chain can be seen running at 40 kDa in SDS-(10%) polyacrylamide gels.

More information can also be found here:

https://www.abcam.com/index.html?pageconfig=resource&rid=11258&pid=11287

Read More

Anja Hoffmann

Abcam Scientific Support

Answered on Dec 10 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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