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  1. Link

    products/secondary-antibodies/goat-chicken-igy-hl-alexa-fluor-488-preadsorbed-ab150173.pdf

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Secondary antibodies anti-Chicken IgY Fluorophore Alexa Fluor® 488
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Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (ab150173)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (43)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (ab150173)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (ab150173)
  • Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (ab150173)

Key features and details

  • Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt

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Overview

  • Product name

    Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed
    See all IgY secondary antibodies
  • Host species

    Goat
  • Target species

    Chicken
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgY and with light chains common to other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.

     

  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Minimal
    cross-reactivity


    Cow, Horse, Human, Mouse, Pig, Rabbit, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to chicken IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Fluorophore
    • Alexa Fluor® 488

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF (1)
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/2000.
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/2000.

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (ab150173)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (ab150173)
    ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab150173 Alexa Fluor® 488 goat anti-chicken IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (ab150173)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (ab150173)
    HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (green) was ab150173 Alexa Fluor® 488 goat anti-chicken IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (ab150173)
    Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (ab150173)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (43)

Publishing research using ab150173? Please let us know so that we can cite the reference in this datasheet.

ab150173 has been referenced in 43 publications.

  • Duan H  et al. Activation of the Notch signaling pathway in the anterior cingulate cortex is involved in the pathological process of neuropathic pain. Pain 162:263-274 (2021). PubMed: 32701650
  • Wegmann S  et al. Persistent repression of tau in the brain using engineered zinc finger protein transcription factors. Sci Adv 7:N/A (2021). PubMed: 33741591
  • Castro-Córdova P  et al. Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection. Nat Commun 12:1140 (2021). PubMed: 33602902
  • Roth E  et al. Behavioural and neurochemical mechanisms underpinning the feeding-suppressive effect of GLP-1/CCK combinatorial therapy. Mol Metab 43:101118 (2021). PubMed: 33221554
  • Nicola C  et al. The Prostate Cancer Therapy Enzalutamide Compared with Abiraterone Acetate/Prednisone Impacts Motivation for Exploration, Spatial Learning and Alters Dopaminergic Transmission in Aged Castrated Mice. Cancers (Basel) 13:N/A (2021). PubMed: 34298734
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Morphological characteristics of p75 neurotrophin receptor-positive cells define a new type of glial cell in the rat dorsal root ganglia

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry
This antibody can be used at 1:500 for IHC.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 26 2021

Map2 expressing radial glial cells

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
This Goat Anti-Chicken secondary antibody (ab150173) is used to probe Map2 primary antibody for 2 hours at room temperature.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Lenin Veeraval

Verified customer

Submitted Nov 11 2020

co-labeling of rabbit anti-CD68 with chicken anti-GFP on mouse nerve tissue

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
the second antibody was used in immunofluorescence co-labeling of CD68 (Texas red) with Chk anti-GFP (ab13970).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 05 2018

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