Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) (ab150172)
Key features and details
- Goat Anti-Chicken IgY H&L (Alexa Fluor® 594)
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt, IHC-FoFr
Overview
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Product name
Goat Anti-Chicken IgY H&L (Alexa Fluor® 594)
See all IgY secondary antibodies -
Host species
Goat -
Target species
Chicken -
Tested applications
Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt, IHC-FoFrmore details -
Immunogen
The details of the immunogen for this antibody are not available.
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Conjugation
Alexa Fluor® 594. Ex: 590nm, Em: 617nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| ICC/IF |
1/200 - 1/1000.
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| ELISA |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| Flow Cyt |
1/2000.
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| IHC-FoFr |
Use at an assay dependent concentration. PubMed: 26755131
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
1/200 - 1/1000. |
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ELISA
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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Flow Cyt
1/2000. |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 26755131 |
Images
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) (ab150172)
ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 10µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150172 Alexa Fluor® 594 goat anti-chicken IgY (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) (ab150172)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab150172 has been referenced in 8 publications.
- Zhu S et al. AAV2-mediated and hypoxia response element-directed expression of bFGF in neural stem cells showed therapeutic effects on spinal cord injury in rats. Cell Death Dis 12:274 (2021). PubMed: 33723238
- Virmani G et al. Subfield-specific effects of chronic mild unpredictable stress on hippocampal astrocytes. Eur J Neurosci 54:5730-5746 (2021). PubMed: 33866634
- Romero-Leguizamón CR et al. Increasing cellular lifespan with a flow system in organotypic culture of the Laterodorsal Tegmentum (LDT). Sci Rep 9:1486 (2019). PubMed: 30728375
- Izquierdo-Lara R et al. Genotype-matched Newcastle disease virus vaccine confers improved protection against genotype XII challenge: The importance of cytoplasmic tails in viral replication and vaccine design. PLoS One 14:e0209539 (2019). PubMed: 31725727
- Materna SC et al. Cardiovascular development and survival require Mef2c function in the myocardial but not the endothelial lineage. Dev Biol 445:170-177 (2019). PubMed: 30521808
- Kumar NN et al. Delivery of immunoglobulin G antibodies to the rat nervous system following intranasal administration: Distribution, dose-response, and mechanisms of delivery. J Control Release 286:467-484 (2018). PubMed: 30081144
- Zhou Q et al. Impairment of PARK14-dependent Ca(2+) signalling is a novel determinant of Parkinson's disease. Nat Commun 7:10332 (2016). IHC-FoFr . PubMed: 26755131
- O'Keeffe GW et al. Region-specific role of growth differentiation factor-5 in the establishment of sympathetic innervation. Neural Dev 11:4 (2016). ICC . PubMed: 26878848