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Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) (ab150172)

Submit a review Submit a question References (10)
Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) (ab150172)
  • Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) (ab150172)

Key features and details

  • Goat Anti-Chicken IgY H&L (Alexa Fluor® 594)
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt, IHC-FoFr

Overview

  • Product name

    Goat Anti-Chicken IgY H&L (Alexa Fluor® 594)
    See all IgY secondary antibodies

  • Host species

    Goat

  • Target species

    Chicken

  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt, IHC-FoFrmore details

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/2000.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 26755131
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/2000.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 26755131

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) (ab150172)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) (ab150172)

    ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 10µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150172 Alexa Fluor® 594 goat anti-chicken IgY (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) (ab150172)
    Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) (ab150172)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (10)

Publishing research using ab150172? Please let us know so that we can cite the reference in this datasheet.

ab150172 has been referenced in 10 publications.

  • Peng Z  et al. Chronic stress-induced depression requires the recruitment of peripheral Th17 cells into the brain. J Neuroinflammation 19:186 (2022). PubMed: 35836182
  • Zhu S  et al. AAV2-mediated and hypoxia response element-directed expression of bFGF in neural stem cells showed therapeutic effects on spinal cord injury in rats. Cell Death Dis 12:274 (2021). PubMed: 33723238
  • Virmani G  et al. Subfield-specific effects of chronic mild unpredictable stress on hippocampal astrocytes. Eur J Neurosci 54:5730-5746 (2021). PubMed: 33866634
  • Sethi P  et al. Automated morphometric analysis with SMorph software reveals plasticity induced by antidepressant therapy in hippocampal astrocytes. J Cell Sci 134:N/A (2021). PubMed: 34137444
  • Romero-Leguizamón CR  et al. Increasing cellular lifespan with a flow system in organotypic culture of the Laterodorsal Tegmentum (LDT). Sci Rep 9:1486 (2019). PubMed: 30728375
  • Izquierdo-Lara R  et al. Genotype-matched Newcastle disease virus vaccine confers improved protection against genotype XII challenge: The importance of cytoplasmic tails in viral replication and vaccine design. PLoS One 14:e0209539 (2019). PubMed: 31725727
  • Materna SC  et al. Cardiovascular development and survival require Mef2c function in the myocardial but not the endothelial lineage. Dev Biol 445:170-177 (2019). PubMed: 30521808
  • Kumar NN  et al. Delivery of immunoglobulin G antibodies to the rat nervous system following intranasal administration: Distribution, dose-response, and mechanisms of delivery. J Control Release 286:467-484 (2018). PubMed: 30081144
  • Zhou Q  et al. Impairment of PARK14-dependent Ca(2+) signalling is a novel determinant of Parkinson's disease. Nat Commun 7:10332 (2016). IHC-FoFr . PubMed: 26755131
  • O'Keeffe GW  et al. Region-specific role of growth differentiation factor-5 in the establishment of sympathetic innervation. Neural Dev 11:4 (2016). ICC . PubMed: 26878848

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