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  1. Link

    products/secondary-antibodies/goat-chicken-igy-hl-alexa-fluor-594-preadsorbed-ab150176.pdf

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Secondary antibodies anti-Chicken IgY Fluorophore Alexa Fluor® 594
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Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (16)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)
  • Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

Key features and details

  • Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 FITC Gold 10nm Gold 25nm HRP HRP PE Texas Red ® Unconjugated Unconjugated

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Overview

  • Product name

    Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed
    See all IgY secondary antibodies
  • Host species

    Goat
  • Target species

    Chicken
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgG and with light chains common to other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.

     

  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Minimal
    cross-reactivity


    Cow, Goat, Horse, Human, Mouse, Pig, Rabbit, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Fluorophore
    • Alexa Fluor® 594

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/2000.
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/2000.

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

    ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 10µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150176 Alexa Fluor® 594 goat anti-chicken IgY (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)
    HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150176 Alexa Fluor® 594 goat anti-chicken IgY (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)
    Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (16)

Publishing research using ab150176? Please let us know so that we can cite the reference in this datasheet.

ab150176 has been referenced in 16 publications.

  • Jiang Y  et al. Basic fibroblast growth factor accelerates myelin debris clearance through activating autophagy to facilitate early peripheral nerve regeneration. J Cell Mol Med 25:2596-2608 (2021). PubMed: 33512767
  • Yan W  et al. Decreased neuronal synaptosome associated protein 29 contributes to poststroke cognitive impairment by disrupting presynaptic maintenance. Theranostics 11:4616-4636 (2021). PubMed: 33754017
  • Huntington TE & Srinivasan R Astrocytic mitochondria in adult mouse brain slices show spontaneous calcium influx events with unique properties. Cell Calcium 96:102383 (2021). PubMed: 33676316
  • Stevens L  et al. A Feasibility Study to Investigate Chemogenetic Modulation of the Locus Coeruleus by Means of Single Unit Activity. Front Neurosci 14:162 (2020). PubMed: 32210746
  • Fleming D  et al. Utilizing glycoside hydrolases to improve the quantitation and visualization of biofilm bacteria. Biofilm 2:100037 (2020). PubMed: 33447822
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Against anti Chicken Ig Y primary antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Application:Immunocytochemistry
Sample: Species: Human
Blocking step:1% BSAin 1xTBS
Primary Antibody: Anti-200 kD Neurofilament Heavy antibody (ab4680), 2 hours Temperature: 4°C, Diluent: 1xTBS with 0.1% BSA
Secondary Antibody: ab150176, Dilution: 1/1000 Incubation time:1 hours at RT Diluent: 1xTBS with 0.1% BSA
Detection method: 594 excitation
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 18 2016

Against anti Chicken Ig Y primary antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Application: ICC
Sample: Species: GFP plasmid overexpression
Blocking step:1X BSA
Primary Antibody: anti-GFP (ab13970), Dilution: 1/200 Incubation time:13 hours at 4°C Diluent: 1xTBS
Secondary Antibody: ab150176, Dilution: 1/400 Incubation time:2 hours at RT Diluent: 1xTBS
Detection method: 594 excitation
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 02 2015

against anti Chicken Ig Y primary antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Application: Western blot

Sample: Species: Mouse Type: Cell lysate - whole cell

Loading amount: 25 µg, Specification: Cardiomyocytes overexpressed with GFP reporter plasmid

Gel Running Conditions: Reduced Denaturing (15% PAGE)

Blocking step: Milk as blocking agent for 1 hour at RT°C Blocking Concentration: 5%


Primary Antibody: anti-GFP (ab13970), Dilution: 1/2000 Incubation time:4 hours at 4°C Diluent: 1xTBS


Secondary Antibody: ab150176, Dilution: 1/5000 Incubation time:2 hours at RT Diluent: 1xTBS


Detection method: 594 excitation


Bands: Specific: ~25 kDa, Non-specific: none
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 02 2015

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