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    products/secondary-antibodies/goat-chicken-igy-hl-alexa-fluor-647-ab150171.pdf

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Secondary antibodies anti-Chicken IgY Fluorophore Alexa Fluor® 647
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Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (ab150171)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (26)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (ab150171)
  • Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (ab150171)

Key features and details

  • Goat Anti-Chicken IgY H&L (Alexa Fluor® 647)
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 FITC Gold 10nm Gold 25nm HRP HRP PE Texas Red ® Unconjugated Unconjugated

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Overview

  • Product name

    Goat Anti-Chicken IgY H&L (Alexa Fluor® 647)
    See all IgY secondary antibodies
  • Host species

    Goat
  • Target species

    Chicken
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Fluorophore
    • Alexa Fluor® 647

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/2000.
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/2000.

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (ab150171)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (ab150171)

    ICC/IF image of ab89984 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 5µg/ml) overnight at +4°C. The secondary antibody (red) was ab150171 Alexa Fluor® 647 goat anti-chicken IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (ab150171)
    Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (ab150171)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (26)

Publishing research using ab150171? Please let us know so that we can cite the reference in this datasheet.

ab150171 has been referenced in 26 publications.

  • Liu H  et al. Catalytically potent and selective clusterzymes for modulation of neuroinflammation through single-atom substitutions. Nat Commun 12:114 (2021). PubMed: 33414464
  • Shin H  et al. 3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics. Nat Commun 12:492 (2021). PubMed: 33479237
  • Amen AM  et al. Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33758097
  • Poon C  et al. Neutrophil recruitment and leukocyte response following focused ultrasound and microbubble mediated blood-brain barrier treatments. Theranostics 11:1655-1671 (2021). PubMed: 33408773
  • Spijkers XM  et al. A directional 3D neurite outgrowth model for studying motor axon biology and disease. Sci Rep 11:2080 (2021). PubMed: 33483540
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Worked perfectly for CLARITY treated tissues

Excellent
Abreviews
Abreviews
abreview image
Application
Other - Do not use
Used as a secondary for anti GFP ab13970. Could image deep into tissue with the AF647 and get very clean images.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Sebastian Alvarado

Verified customer

Submitted May 04 2016

Worked well!

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Does the trick just fine.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Sebastian Alvarado

Verified customer

Submitted Dec 29 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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