Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (ab150171)
Key features and details
- Goat Anti-Chicken IgY H&L (Alexa Fluor® 647)
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Goat Anti-Chicken IgY H&L (Alexa Fluor® 647)
See all IgY secondary antibodies -
Host species
Goat -
Target species
Chicken -
Tested applications
Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details -
Immunogen
The details of the immunogen for this antibody are not available.
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Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| ICC/IF |
1/200 - 1/1000.
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| ELISA |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| Flow Cyt |
1/2000.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
1/200 - 1/1000. |
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ELISA
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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Flow Cyt
1/2000. |
Images
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (ab150171)
ICC/IF image of ab89984 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 5µg/ml) overnight at +4°C. The secondary antibody (red) was ab150171 Alexa Fluor® 647 goat anti-chicken IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (ab150171)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab150171 has been referenced in 26 publications.
- Liu H et al. Catalytically potent and selective clusterzymes for modulation of neuroinflammation through single-atom substitutions. Nat Commun 12:114 (2021). PubMed: 33414464
- Shin H et al. 3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics. Nat Commun 12:492 (2021). PubMed: 33479237
- Amen AM et al. Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33758097
- Poon C et al. Neutrophil recruitment and leukocyte response following focused ultrasound and microbubble mediated blood-brain barrier treatments. Theranostics 11:1655-1671 (2021). PubMed: 33408773
- Spijkers XM et al. A directional 3D neurite outgrowth model for studying motor axon biology and disease. Sci Rep 11:2080 (2021). PubMed: 33483540