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Goat Anti-Chicken IgY H&L (Biotin) preadsorbed (ab7117)

Submit a review Q&A (1)

Key features and details

  • Goat Anti-Chicken IgY H&L (Biotin) preadsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Goat Anti-Chicken IgY H&L (Biotin) preadsorbed
    See all IgY secondary antibodies

  • Host species

    Goat

  • Target species

    Chicken

  • Tested applications

    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

  • Minimal
    cross-reactivity


    Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details

  • Immunogen

    Full length native chicken IgY (purified).

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    IgY fraction

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

  • Clonality

    Polyclonal

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
Notes
Application notes
Dot: Use at an assay dependant dilution.
ELISA: 1/8000 - 1/36000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab7117? Please let us know so that we can cite the reference in this datasheet.

ab7117 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Thanks for your help, but I am still uncertain about my question regarding the addition of glycerol. "The storage instructions stated to add 50% glycerol before freezing. Should this be 50% of the current volume or a final concentration of 50%".

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Answer

Thank you for your message. I have double-checked with my colleagues and actually we have reconstituted this antibody and already added 50% glycerol to the buffer. This means that for long term storage, you do not need to add anything just aliquot the product and keep it at -20°C. We have corrected our on-line datasheet in order to avoid more confusion. Thank you again for bringing this matter to our attention.

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