Goat Anti-Chicken IgY H&L (Cy2 ®) preadsorbed (ab6960)
Key features and details
- Goat Anti-Chicken IgY H&L (Cy2 ®) preadsorbed
- Conjugation: Cy2 ®. Ex: 489nm, Em: 505nm
- Host species: Goat
- Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA
Related conjugates and formulations
Overview
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Product name
Goat Anti-Chicken IgY H&L (Cy2 ®) preadsorbed
See all IgY secondary antibodies -
Host species
Goat -
Target species
Chicken -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details -
Minimal
cross-reactivity
Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details -
Immunogen
Full length native Chicken IgY (purified).
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Conjugation
Cy2 ®. Ex: 489nm, Em: 505nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 10% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 2.7 moles Cy2 per mole of Goat IgG -
Clonality
Polyclonal -
General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/5000.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent dilution.
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ELISA |
1/10000 - 1/50000.
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Notes |
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ICC/IF
1/1000 - 1/5000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent dilution. |
ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (5)
ab6960 has been referenced in 5 publications.
- Okujeni S & Egert U Self-organization of modular network architecture by activity-dependent neuronal migration and outgrowth. Elife 8:N/A (2019). PubMed: 31526478
- Anastasiades PG et al. GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex. Nat Commun 7:10584 (2016). IHC-FoFr . PubMed: 26843463
- Stroebele E & Erives A Integration of Orthogonal Signaling by the Notch and Dpp Pathways in Drosophila. Genetics 203:219-40 (2016). PubMed: 26975664
- Meyer RE et al. A single mutation results in diploid gamete formation and parthenogenesis in a Drosophila yemanuclein-alpha meiosis I defective mutant. BMC Genet 11:104 (2010). ICC/IF . PubMed: 21080953
- Dinger TC et al. Androgenetic embryonic stem cells form neural progenitor cells in vivo and in vitro. Stem Cells 26:1474-83 (2008). IHC-Fr . PubMed: 18369101