Goat Anti-Chicken IgY H&L (Cy3 ®) preadsorbed (ab97145)
Key features and details
- Goat Anti-Chicken IgY H&L (Cy3 ®) preadsorbed
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Goat Anti-Chicken IgY H&L (Cy3 ®) preadsorbed
See all IgY secondary antibodies -
Host species
Goat -
Target species
Chicken -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.
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Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Minimal
cross-reactivity
Cow, Horse, Human, Mouse, Pig, Rabbit, Rat more details -
Immunogen
Other Immunogen Type corresponding to Chicken IgY.
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Conjugation
Cy3 ®. Ex: 552nm, Em: 565nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3 ®. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50 - 1/500.
|
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| ICC/IF |
1/50 - 1/500.
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| Flow Cyt |
1/100 - 1/1000.
|
| Notes |
|---|
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IHC-P
1/50 - 1/500. |
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ICC/IF
1/50 - 1/500. |
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Flow Cyt
1/100 - 1/1000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (8)
ab97145 has been referenced in 8 publications.
- Liška K et al. High Sensitivity of the Circadian Clock in the Hippocampal Dentate Gyrus to Glucocorticoid- and GSK3-Beta-Dependent Signals. Neuroendocrinology 112:384-398 (2022). PubMed: 34111876
- Shi SQ et al. Circadian Rhythms and Sleep Are Dependent Upon Expression Levels of Key Ubiquitin Ligase Ube3a. Front Behav Neurosci 16:837523 (2022). PubMed: 35401134
- Chaudhari K et al. Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion. Elife 10:N/A (2021). PubMed: 33843588
- Singal CMS et al. Role of EphrinA3 in HIV-1 Neuropathogenesis. ASN Neuro 13:17590914211044359 (2021). PubMed: 34618621
- Gong J et al. Translocator Protein Ligand Protects against Neurodegeneration in the MPTP Mouse Model of Parkinsonism. J Neurosci 39:3752-3769 (2019). PubMed: 30796158
- García-Fernández E et al. Membrane Microdomain Disassembly Inhibits MRSA Antibiotic Resistance. Cell 171:1354-1367.e20 (2017). PubMed: 29103614
- Huang H et al. A Novel Approach for Meniscal Regeneration Using Kartogenin-Treated Autologous Tendon Graft. Am J Sports Med 45:3289-3297 (2017). PubMed: 28859517
- Papadakis M et al. Tsc1 (hamartin) confers neuroprotection against ischemia by inducing autophagy. Nat Med 19:351-7 (2013). PubMed: 23435171
