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Goat Anti-Chicken IgY H&L (Cy3 ®) preadsorbed (ab97145)

Submit a review Q&A (2)References (5)

Key features and details

  • Goat Anti-Chicken IgY H&L (Cy3 ®) preadsorbed
  • Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ICC/IF, Flow Cyt

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 FITC Gold 10nm Gold 25nm HRP HRP PE Texas Red ® Unconjugated Unconjugated

Overview

  • Product name

    Goat Anti-Chicken IgY H&L (Cy3 ®) preadsorbed
    See all IgY secondary antibodies

  • Host species

    Goat

  • Target species

    Chicken

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.

     

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details

  • Minimal
    cross-reactivity


    Cow, Horse, Human, Mouse, Pig, Rabbit, Rat more details

  • Immunogen

    Other Immunogen Type corresponding to Chicken IgY.

  • Conjugation

    Cy3 ®. Ex: 552nm, Em: 565nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3 ®.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50 - 1/500.
ICC/IF
1/50 - 1/500.
Flow Cyt
1/100 - 1/1000.
Notes
IHC-P
1/50 - 1/500.
ICC/IF
1/50 - 1/500.
Flow Cyt
1/100 - 1/1000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (5)

Publishing research using ab97145? Please let us know so that we can cite the reference in this datasheet.

ab97145 has been referenced in 5 publications.

  • Chaudhari K  et al. Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion. Elife 10:N/A (2021). PubMed: 33843588
  • Gong J  et al. Translocator Protein Ligand Protects against Neurodegeneration in the MPTP Mouse Model of Parkinsonism. J Neurosci 39:3752-3769 (2019). PubMed: 30796158
  • García-Fernández E  et al. Membrane Microdomain Disassembly Inhibits MRSA Antibiotic Resistance. Cell 171:1354-1367.e20 (2017). PubMed: 29103614
  • Huang H  et al. A Novel Approach for Meniscal Regeneration Using Kartogenin-Treated Autologous Tendon Graft. Am J Sports Med 45:3289-3297 (2017). PubMed: 28859517
  • Papadakis M  et al. Tsc1 (hamartin) confers neuroprotection against ischemia by inducing autophagy. Nat Med 19:351-7 (2013). PubMed: 23435171

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Auto-fluorescence in the tissue. Sudan black treatment gave tissue a red tint.

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Answer



As we discussed, please send me the images of the staining. I would suggest using a positive control and confirming the GFP transfection, but trying a fluorophore from an infrared channel where the auto-fluorescence is lower may be a good solution.

Read More

Question

Customer kindly contacted us to discuss use of this product in IHC-P, issues with autofluorescence and secondary antibody choices.

Read More
Answer

Thank you for contacting us.

I have included with this email 2 documents about autofluorescence and tips for reducing this as requested.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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