Goat Anti-Chicken IgY H&L (Cy5 ®) preadsorbed (ab97147)
Key features and details
- Goat Anti-Chicken IgY H&L (Cy5 ®) preadsorbed
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Goat Anti-Chicken IgY H&L (Cy5 ®) preadsorbed
See all IgY secondary antibodies -
Host species
Goat -
Target species
Chicken -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.
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Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Minimal
cross-reactivity
Cow, Horse, Human, Mouse, Pig, Rabbit, Rat more details -
Immunogen
Other Immunogen Type corresponding to Chicken IgY.
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Conjugation
Cy5 ®. Ex: 650nm, Em: 667nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy5 ®. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/500.
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ICC/IF | (1) |
1/50 - 1/500.
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Flow Cyt |
1/100 - 1/1000.
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Notes |
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IHC-P
1/50 - 1/500. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/100 - 1/1000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab97147 has been referenced in 8 publications.
- Aiken J et al. TUBA1A mutations identified in lissencephaly patients dominantly disrupt neuronal migration and impair dynein activity. Hum Mol Genet 28:1227-1243 (2019). PubMed: 30517687
- Bezhaeva T et al. Contribution of bone marrow-derived cells to in situ engineered tissue capsules in a rat model of chronic kidney disease. Biomaterials 194:47-56 (2019). PubMed: 30580195
- Florio F et al. Sustained Expression of Negative Regulators of Myelination Protects Schwann Cells from Dysmyelination in a Charcot-Marie-Tooth 1B Mouse Model. J Neurosci 38:4275-4287 (2018). PubMed: 29610440
- Hawkins SJ et al. Nanoparticle-induced neuronal toxicity across placental barriers is mediated by autophagy and dependent on astrocytes. Nat Nanotechnol 13:427-433 (2018). PubMed: 29610530
- Di Spiezio A et al. The LepR-mediated leptin transport across brain barriers controls food reward. Mol Metab 8:13-22 (2018). IHC-P ; Mouse . PubMed: 29254602
- Casie Chetty S et al. Vegf signaling promotes vascular endothelial differentiation by modulating etv2 expression. Dev Biol 424:147-161 (2017). PubMed: 28279709
- Müller-Fielitz H et al. Tanycytes control the hormonal output of the hypothalamic-pituitary-thyroid axis. Nat Commun 8:484 (2017). PubMed: 28883467
- Su S et al. Effects of GnRH immunization on the reproductive axis and thymulin. J Endocrinol 226:93-102 (2015). PubMed: 26016747