Goat Anti-Chicken IgY H&L (DyLight® 550) (ab96948)
Key features and details
- Goat Anti-Chicken IgY H&L (DyLight® 550)
- Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, IHC-P
Related conjugates and formulations
Overview
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Product name
Goat Anti-Chicken IgY H&L (DyLight® 550)
See all IgY secondary antibodies -
Host species
Goat -
Target species
Chicken -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details -
Conjugation
DyLight® 550. Ex: 562nm, Em: 576nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
DyLight® 550 replaces DyLight® 549. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/50 - 1/200.
|
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| ICC/IF |
1/50 - 1/500.
|
|
| IHC-P |
1/50 - 1/500.
DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
| Notes |
|---|
|
Flow Cyt
1/50 - 1/200. |
|
ICC/IF
1/50 - 1/500. |
|
IHC-P
1/50 - 1/500. DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
Images
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Goat Anti-Chicken IgY H&L (DyLight® 550) (ab96948)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab96948 has been referenced in 3 publications.
- Ng L et al. Celastrol Downmodulates Alpha-Synuclein-Specific T Cell Responses by Mediating Antigen Trafficking in Dendritic Cells. Front Immunol 13:833515 (2022). PubMed: 35309340
- Silva DF et al. Microbial BMAA elicits mitochondrial dysfunction, innate immunity activation, and Alzheimer's disease features in cortical neurons. J Neuroinflammation 17:332 (2020). PubMed: 33153477
- Ghidinelli M et al. Laminin 211 inhibits protein kinase A in Schwann cells to modulate neuregulin 1 type III-driven myelination. PLoS Biol 15:e2001408 (2017). IHC ; Mouse . PubMed: 28636612
