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  1. Link

    products/secondary-antibodies/goat-chicken-igy-hl-fitc-ab6873.pdf

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Secondary antibodies anti-Chicken IgY Fluorophore FITC
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Goat Anti-Chicken IgY H&L (FITC) (ab6873)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (4)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (FITC) (ab6873)
  • Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (FITC) (ab6873)

Key features and details

  • Goat Anti-Chicken IgY H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Goat
  • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 Gold 10nm Gold 25nm HRP HRP PE Texas Red ® Unconjugated Unconjugated

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Overview

  • Product name

    Goat Anti-Chicken IgY H&L (FITC)
    See all IgY secondary antibodies
  • Host species

    Goat
  • Target species

    Chicken
  • Tested applications

    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    chicken IgY whole molecule

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Goat IgG
  • Clonality

    Polyclonal
  • Research areas

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Fluorophore
    • FITC

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ICC/IF (1)
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.
Notes
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (FITC) (ab6873)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (FITC) (ab6873)
    Ab6873 was used at dilution 1/500 with the primary antibody ab39376 in ICC/IF. See the review on ab39376.
  • Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (FITC) (ab6873)
    Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (FITC) (ab6873)
    Ab6873 was used at dilution 1/500 with the primary antibody ab13991 in IHC-Fr. See the review on ab13991.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (4)

Publishing research using ab6873? Please let us know so that we can cite the reference in this datasheet.

ab6873 has been referenced in 4 publications.

  • Wu H  et al. Ubiquitination Is Essential for Avibirnavirus Replication by Supporting VP1 Polymerase Activity. J Virol 93:N/A (2019). PubMed: 30429342
  • Munera D  et al. Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4. Nat Commun 5:3080 (2014). PubMed: 24445323
  • Millet YA  et al. Insights into Vibrio cholerae intestinal colonization from monitoring fluorescently labeled bacteria. PLoS Pathog 10:e1004405 (2014). IF ; Human . PubMed: 25275396
  • Sully K  et al. The mTOR inhibitor rapamycin opposes carcinogenic changes to epidermal Akt1/PKBa isoform signaling. Oncogene : (2012). PubMed: 22890326

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

GFAP staining in primary rat astrocytes

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Antibody was diluted 1/1000 in PBS with 1% goat serum and incubated 1h at RT
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 29 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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