Goat Anti-Chicken IgY H&L (FITC) (ab6873)
Key features and details
- Goat Anti-Chicken IgY H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA
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Overview
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Product name
Goat Anti-Chicken IgY H&L (FITC)
See all IgY secondary antibodies -
Host species
Goat -
Target species
Chicken -
Tested applications
Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details -
Immunogen
chicken IgY whole molecule
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Tripotassium orthophosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Goat IgG -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent dilution.
|
|
| ICC/IF | (1) |
1/1000 - 1/5000.
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| ELISA |
1/10000 - 1/50000.
|
| Notes |
|---|
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent dilution. |
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ELISA
1/10000 - 1/50000. |
Images
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (FITC) (ab6873)
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Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (FITC) (ab6873)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab6873 has been referenced in 4 publications.
- Wu H et al. Ubiquitination Is Essential for Avibirnavirus Replication by Supporting VP1 Polymerase Activity. J Virol 93:N/A (2019). PubMed: 30429342
- Munera D et al. Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4. Nat Commun 5:3080 (2014). PubMed: 24445323
- Millet YA et al. Insights into Vibrio cholerae intestinal colonization from monitoring fluorescently labeled bacteria. PLoS Pathog 10:e1004405 (2014). IF ; Human . PubMed: 25275396
- Sully K et al. The mTOR inhibitor rapamycin opposes carcinogenic changes to epidermal Akt1/PKBa isoform signaling. Oncogene : (2012). PubMed: 22890326