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  1. Link

    products/secondary-antibodies/goat-donkey-igg-hl-hrp-ab6988.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Donkey IgG H&L (HRP) (ab6988)

  • Datasheet
Reviews (1) Submit a question References (2)

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Key features and details

  • Goat Anti-Donkey IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Suitable for: ICC/IF, WB, ELISA, Immunomicroscopy, Dot blot, IHC-P, IHC-Fr

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Goat Anti-Donkey IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Donkey
  • Tested applications

    Suitable for: ICC/IF, WB, ELISA, Immunomicroscopy, Dot blot, IHC-P, IHC-Frmore details
  • Immunogen

    Full length native donkey IgG (purified).

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Horseradish Peroxidase (HRP).
  • Conjugation notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using donkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality

    Polyclonal
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Donkey
    • IgG
    • Enzyme
    • HRP

Associated products

  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA (1)
Immunomicroscopy
Dot blot
IHC-P
IHC-Fr
Notes
Application notes
Dot: Use at an assay dependant dilution.
ELISA: 1/20000 - 1/200000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (2)

Publishing research using ab6988? Please let us know so that we can cite the reference in this datasheet.

ab6988 has been referenced in 2 publications.

  • Wong J  et al. Sequencing B cell receptors from ferrets (Mustela putorius furo). PLoS One 15:e0233794 (2020). PubMed: 32470013
  • Ashrafi GH  et al. Vaccination of sarcoid-bearing donkeys with chimeric virus-like particles of bovine papillomavirus type 1. J Gen Virol 89:148-57 (2008). PubMed: 18089738

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Works well as a secondary Ab in ELISA

Excellent
Abreviews
Abreviews
abreview image
Application
ELISA
Used to estimate the coverage of a well (96-wellplate) coated with a mouse a-CD28ab, previously incubated with a donkey-raised a-mouse ab. Working dilution 1:5000 in 1% BSA. Detection was possible even in tiny amounts of the target and consistent in following replicates.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 16 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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