Goat F(ab) Anti-Mouse IgG H&L (HRP) (ab6823)
Key features and details
- Goat F(ab) Anti-Mouse IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC
Related conjugates and formulations
Overview
-
Product name
Goat F(ab) Anti-Mouse IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. It was tested against anti goat F(c) fragment to verify no contaminating goat F(c) was present in the fraction. No reaction was observed against anti-Papain or anti-Goat IgG F(c). -
Tested applications
Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
Immunogen
Mouse IgG whole molecule
-
Conjugation
HRP
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 0.0014% Zinc chloride
BSA is IgG and protease free -
Concentration information loading... -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. -
Conjugation notes
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/500 - 1/2500.
|
|
| Dot blot |
Use at an assay dependent dilution.
|
|
| WB |
1/500 - 1/2500.
|
|
| ELISA |
1/10000 - 1/100000.
|
|
| Electron Microscopy |
Use at an assay dependent dilution.
|
|
| IHC |
Use at an assay dependent dilution.
|
| Notes |
|---|
|
ICC/IF
1/500 - 1/2500. |
|
Dot blot
Use at an assay dependent dilution. |
|
WB
1/500 - 1/2500. |
|
ELISA
1/10000 - 1/100000. |
|
Electron Microscopy
Use at an assay dependent dilution. |
|
IHC
Use at an assay dependent dilution. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (37)
ab6823 has been referenced in 37 publications.
- Zhang J et al. Comparison of the effect of rotenone and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine on inducing chronic Parkinson's disease in mouse models. Mol Med Rep 25:N/A (2022). PubMed: 35039876
- Escalera A et al. Mutations in SARS-CoV-2 variants of concern link to increased spike cleavage and virus transmission. Cell Host Microbe 30:373-387.e7 (2022). PubMed: 35150638
- van der Heide V et al. Limited extent and consequences of pancreatic SARS-CoV-2 infection. Cell Rep 38:110508 (2022). PubMed: 35247306
- Li H et al. Gene therapy of yeast NDI1 on mitochondrial complex I dysfunction in rotenone-induced Parkinson's disease models in vitro and vivo. Mol Med 28:29 (2022). PubMed: 35255803
- Rathnasinghe R et al. Characterization of SARS-CoV-2 Spike mutations important for infection of mice and escape from human immune sera. Nat Commun 13:3921 (2022). PubMed: 35798721
