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    products/secondary-antibodies/goat-fab-mouse-igg-hl-hrp-ab6823.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat F(ab) Anti-Mouse IgG H&L (HRP) (ab6823)

  • Datasheet
Submit a review Q&A (1)References (31)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab) Anti-Mouse IgG H&L (HRP) (ab6823)

    Key features and details

    • Goat F(ab) Anti-Mouse IgG H&L (HRP)
    • Conjugation: HRP
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC

    Conjugates logo Related conjugates and formulations

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    Overview

    • Product name

      Goat F(ab) Anti-Mouse IgG H&L (HRP)
      See all IgG secondary antibodies
    • Host species

      Goat
    • Target species

      Mouse
    • Specificity

      Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. It was tested against anti goat F(c) fragment to verify no contaminating goat F(c) was present in the fraction. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
    • Tested applications

      Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
    • Immunogen

      Mouse IgG whole molecule

    • Conjugation

      HRP

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Constituent: 0.0014% Zinc chloride

      BSA is IgG and protease free
    • Concentration information loading...
    • Purification notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
    • Conjugation notes

      Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Mouse
      • IgG
      • Enzyme
      • HRP

    Associated products

    • Substrate reagent

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6823 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/500 - 1/2500.
    Dot blot
    Use at an assay dependent dilution.
    WB
    1/500 - 1/2500.
    ELISA
    1/10000 - 1/100000.
    Electron Microscopy
    Use at an assay dependent dilution.
    IHC
    Use at an assay dependent dilution.
    Notes
    ICC/IF
    1/500 - 1/2500.
    Dot blot
    Use at an assay dependent dilution.
    WB
    1/500 - 1/2500.
    ELISA
    1/10000 - 1/100000.
    Electron Microscopy
    Use at an assay dependent dilution.
    IHC
    Use at an assay dependent dilution.

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab) Anti-Mouse IgG H&L (HRP) (ab6823)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab) Anti-Mouse IgG H&L (HRP) (ab6823)
      Ab6823 was used with the primary antibody ab15263 in IHC-P. See Abreview on ab15263.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (31)

    Publishing research using ab6823? Please let us know so that we can cite the reference in this datasheet.

    ab6823 has been referenced in 31 publications.

    • Zhang J  et al. Comparison of the effect of rotenone and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine on inducing chronic Parkinson's disease in mouse models. Mol Med Rep 25:N/A (2022). PubMed: 35039876
    • Escalera A  et al. Mutations in SARS-CoV-2 variants of concern link to increased spike cleavage and virus transmission. Cell Host Microbe 30:373-387.e7 (2022). PubMed: 35150638
    • van der Heide V  et al. Limited extent and consequences of pancreatic SARS-CoV-2 infection. Cell Rep 38:110508 (2022). PubMed: 35247306
    • Li H  et al. Gene therapy of yeast NDI1 on mitochondrial complex I dysfunction in rotenone-induced Parkinson's disease models in vitro and vivo. Mol Med 28:29 (2022). PubMed: 35255803
    • Nikiforova N  et al. CM-Dil Staining and SEC of Plasma as an Approach to Increase Sensitivity of Extracellular Nanovesicles Quantification by Bead-Assisted Flow Cytometry. Membranes (Basel) 11:N/A (2021). PubMed: 34357175
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Hello, I would like to know if this product exist without HRP?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 01 2014

    Answer

    The product you are looking for is ab6668, Goat F(ab) polyclonal Secondary Antibody to Mouse IgG without HRP.

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Apr 01 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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