Goat F(ab) Anti-Rabbit IgG H&L (Texas Red ®) (ab7052)
Key features and details
- Goat F(ab) Anti-Rabbit IgG H&L (Texas Red ®)
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISA
Related conjugates and formulations
Overview
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Product name
Goat F(ab) Anti-Rabbit IgG H&L (Texas Red ®)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c). -
Tested applications
Suitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details -
Immunogen
Rabbit IgG whole molecule
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Conjugation
Texas Red ®. Ex: 596nm, Em: 620nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, Papain digestion and chromatographic separation. -
Conjugation notes
Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.0 moles Texas Red per mole of Goat IgG Fab -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
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| Flow Cyt |
1/500 - 1/2500.
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| Immunomicroscopy |
Use at an assay dependent concentration.
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| ELISA |
1/10000 - 1/50000.
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| Notes |
|---|
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ICC/IF
1/1000 - 1/5000. |
|
Flow Cyt
1/500 - 1/2500. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
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ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab7052 has been referenced in 3 publications.
- Wang S et al. PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection. Nat Commun 13:846 (2022). PubMed: 35149691
- Zhang H et al. DEAD-Box Helicase 18 Counteracts PRC2 to Safeguard Ribosomal DNA in Pluripotency Regulation. Cell Rep 30:81-97.e7 (2020). PubMed: 31914400
- Autour A et al. Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells. Nat Commun 9:656 (2018). PubMed: 29440634
