Goat F(ab')2 Anti-Human IgG (Fab')2 (ab98531)
Key features and details
- Goat F(ab')2 Anti-Human IgG (Fab')2
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Related conjugates and formulations
Overview
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Product name
Goat F(ab')2 Anti-Human IgG (Fab')2
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with F(ab')2 fragments of IgG from other species.
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Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Immunogen
Other Immunogen Type corresponding to Human IgG.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab98531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent dilution.
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| IHC-P |
1/200 - 1/2000.
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| ELISA |
1/100 - 1/30000. Coating: Use 1/100 - 1/500 dilution; Primary: Use 1/1000 - 1/30000 dilution.
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| WB |
1/1000 - 1/30000. Colorimetric: Use 1/1000 - 1/10000 dilution; Chemiluminescent: Use 1/1000 - 1/30000 dilution.
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| Notes |
|---|
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ICC
Use at an assay dependent dilution. |
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IHC-P
1/200 - 1/2000. |
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ELISA
1/100 - 1/30000. Coating: Use 1/100 - 1/500 dilution; Primary: Use 1/1000 - 1/30000 dilution. |
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WB
1/1000 - 1/30000. Colorimetric: Use 1/1000 - 1/10000 dilution; Chemiluminescent: Use 1/1000 - 1/30000 dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab98531 has been referenced in 1 publication.
- Vanni I et al. Isolation of infectious, non-fibrillar and oligomeric prions from a genetic prion disease. Brain 143:1512-1524 (2020). PubMed: 32303068