Goat F(ab')2 Anti-Human IgG - Fc (DyLight® 650), pre-adsorbed (ab98593)
Key features and details
- Goat F(ab')2 Anti-Human IgG - Fc (DyLight® 650), pre-adsorbed
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Goat F(ab')2 Anti-Human IgG - Fc (DyLight® 650), pre-adsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Minimal
cross-reactivity
Mouse, Rat more details -
Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 650. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
DyLight® 650 replaces DyLight® 649. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab98593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
|
| ICC/IF |
1/50 - 1/500.
|
|
| Flow Cyt |
1/50 - 1/200.
|
| Notes |
|---|
|
IHC-P
1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
|
ICC/IF
1/50 - 1/500. |
|
Flow Cyt
1/50 - 1/200. |
Images
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Goat F(ab')2 Anti-Human IgG - Fc (DyLight® 650), pre-adsorbed (ab98593)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab98593 has been referenced in 2 publications.
- Zhong Z et al. Development of a bispecific antibody targeting PD-L1 and TIGIT with optimal cytotoxicity. Sci Rep 12:18011 (2022). PubMed: 36289396
- Langers I et al. Natural killer and dendritic cells collaborate in the immune response induced by the vaccine against uterine cervical cancer. Eur J Immunol 44:3585-95 (2014). PubMed: 25229656
