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Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed (ab5886)

Submit a review Q&A (1)References (3)

Key features and details

  • Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy

Conjugates logo Related conjugates and formulations

DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC HRP HRP PE TRITC Unconjugated

Overview

  • Product name

    Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgGF(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

  • Tested applications

    Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details

  • Minimal
    cross-reactivity


    Cow, Horse, Human, Rabbit, Rat, Sheep more details

  • Immunogen

    Mouse IgG F(ab)2 fragment

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • Conjugation notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent dilution.
WB
1/2000 - 1/10000.
ELISA
1/20000 - 1/100000.
IHC
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
Notes
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent dilution.
WB
1/2000 - 1/10000.
ELISA
1/20000 - 1/100000.
IHC
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (3)

Publishing research using ab5886? Please let us know so that we can cite the reference in this datasheet.

ab5886 has been referenced in 3 publications.

  • Di Persio S  et al. Single-cell RNA-seq unravels alterations of the human spermatogonial stem cell compartment in patients with impaired spermatogenesis. Cell Rep Med 2:100395 (2021). PubMed: 34622232
  • Onishi Y  et al. Relationship between lamellar sensory corpuscles distributed along the upper arm's deep arteries and pulsating sensation of blood vessels. J Anat 239:101-110 (2021). PubMed: 33527396
  • Schneider F  et al. Serum and intratesticular inhibin B, AMH, and spermatogonial numbers in trans women at gender-confirming surgery: An observational study. Andrology 9:1781-1789 (2021). PubMed: 34085780

Customer reviews and Q&As

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Submit a review Submit a question

Question

Hi,



I'm looking for a biotin labelled F(ab')2 antimouse antibody for peroxidase immunohistochemistry (lung tissue --> first antibody = monoclonal antibody IgG1 that detects adducts of hypoxyprobe). ABCAM provides three different antibodies; ab98657, ab98756 and ab5886. How do these products differ from each other? And what does pre-adsorbed means?



Many thanks in advance for your help!

Read More
Answer

Thank you for your enquiry and your interest in our products.
Although, all these three secondary antibodies (ab5886, ab98756, ab98657) recognize mouse IgG and were raised in goat, they are from different sources. The tested application/species/concentration may vary.
Pre-adsorbed antibodies (ab5886, ab98756) are prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. This means that they only recognize mouse IgG and any cross-reactivity with other species is minimal.
I hope this helps and if I can assist further, please do not hesitate to contact me.

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