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Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (DyLight® 550), pre-adsorbed (ab98758)

Submit a review Q&A (3)References (1)
Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (DyLight® 550), pre-adsorbed (ab98758)

    Key features and details

    • Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (DyLight® 550), pre-adsorbed
    • Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: Flow Cyt, ICC/IF, IHC-P

    Conjugates logo Related conjugates and formulations

    Biotin DyLight® 488 DyLight® 594 DyLight® 650 FITC HRP HRP PE TRITC Unconjugated

    Overview

    • Product name

      Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (DyLight® 550), pre-adsorbed
      See all IgG secondary antibodies

    • Host species

      Goat

    • Target species

      Mouse

    • Tested applications

      Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details

    • Minimal
      cross-reactivity


      Human, Rat more details

    • Conjugation

      DyLight® 550. Ex: 562nm, Em: 576nm

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: PBS, 0.2% BSA
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 550.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • General notes

      DyLight® 550 replaces DyLight® 549.

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab98758 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    1/50 - 1/200.
    ICC/IF
    1/50 - 1/500.
    IHC-P
    1/50 - 1/500.
    Notes
    Flow Cyt
    1/50 - 1/200.
    ICC/IF
    1/50 - 1/500.
    IHC-P
    1/50 - 1/500.

    Images

    • Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (DyLight® 550), pre-adsorbed (ab98758)
      Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (DyLight® 550), pre-adsorbed (ab98758)
      Emission spectra of DyLight® fluorochromes available in our catalog.
      Line colors represent the approximate visible colors of the wavelength maxima.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab98758? Please let us know so that we can cite the reference in this datasheet.

    ab98758 has been referenced in 1 publication.

    • Stebbing J  et al. JAK inhibition reduces SARS-CoV-2 liver infectivity and modulates inflammatory responses to reduce morbidity and mortality. Sci Adv 7:N/A (2021). PubMed: 33187978

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Inquiry: Our customers wishes to use either ab98763 or ab5889 or ab98758 in Flow Cyt with channel 2. Kindly advise which one of the following is preferable (DyLight 550 or PE). Thanks in advance for the assistance and reply. Have a nice day

    Read More
    Answer

    Thank you for contacting us.
    The user will have to verify the excitation laser and emission detection filters of their instrument.
    Dylight 550 properties: Excitation at 562 nm, emission at 576 nm
    PE properties: Excitation at 488 nm, emission at 578 nm
    Typically, for channel 2 detection (filter = 585/40 nm) PE is a very bright fluorophore with large stokes shift (excitation with 488 laser). Dylight 550 is spectrally similar to AlexaFluor 546, AlexaFluor 555, Cy3, and TRITC.
    We tend to choose PE conjugates for channel 2 detection.
    I hope this helps and if we can assist further, please do not hesitate to contact us.

    Read More

    Question

    Thank you very much! In this way I should be able to image the aggrecan in green and the collagen II in red, is this correct? Is there any kind of discount or special price for this package?

    Read More
    Answer

    It is my pleasure to help.

    In this particular group, aggrecan would fluoresce in green while collagen II would be red. We offer other dyes athttps://www.abcam.com/index.html?pageconfig=resource&rid=12956should you wish to change those colors.

    We do offer discounts on purchases of five items or more and offer a number of promotions. Our customer service representatives will be able to give you more detailed information about our current promotions and discounts or you may follow the link here:https://www.abcam.com/index.html?pageconfig=resource&rid=14853

    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

    Question

    Hello, I need to image collagen type II and aggrecan from mouse within the same samples. The laser that I have available are 458, 488, 514, 543, 633. Which combination of antibodies (primary and secondary) do you recommend that might work nicely together? Many thanks for your assistance.

    Read More
    Answer

    Thank you for contacting us.

    We presently have only 1 product against mouse aggrecan, ab36861. This is a rabbit polyclonal. In ICC and appropriate secondary would be anti-Rabbit IgG with a fluorescent tag which could be excited by your lasers. A product such as ab96899,Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488),https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-pre-adsorbed-ab96899.html

    We currently have 20 products against collagen II. However taking into account that this will have to be used in mouse tissue in conjunction with an anti-aggrecan rabbit polyclonal we can limit this to ab3092Anti-Collagen II antibody [5B2.5]. This is a mouse monoclonal antibody of isotype IgG2a that has been tested inIHC-Fr,IHC-P,WB and IF. An appropriate secondary should preferably be a F(ab) fragment as mouse on mouse staining can be difficult, please see this link for more information about mouse on mouse staining,https://www.abcam.com/index.html?pageconfig=resource&rid=11465. One such product would be ourGoat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2 (DyLight® 550), pre-adsorbed,https://www.abcam.com/Goat-F-ab-2-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fab-2-DyLight-550-pre-adsorbed-ab98758.html

    Abcam also offers a number of ICC and IHC reagents. As both of the secondary antibodies listed above are raised in goat, we recommend using a 5-10% Goat serum as your blocking reagent and antibody incubation solution, ab7481https://www.abcam.com/Goat-Serum-ab7481.html. We, also offer buffers and antigen retrieval solutions,https://www.abcam.com/index.html?pageconfig=resource&rid=12854, as well as mounting media for fluorescent staining ab103748,https://www.abcam.com/BrightMount-Plus-25ml-Aqueous-Mounting-Medium-Anti-fading-for-Fluorescent-Staining-ab103748.html.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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