Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (FITC), pre-adsorbed (ab5883)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (FITC), pre-adsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Flow Cyt, ELISA, Immunomicroscopy
Related conjugates and formulations
Overview
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Product name
Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (FITC), pre-adsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against Mouse IgG F(c), anti-Pepsin, anti-Goat IgG F(ab’)2 or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. -
Tested applications
Suitable for: ICC/IF, Flow Cyt, ELISA, Immunomicroscopymore details -
Minimal
cross-reactivity
Cow, Horse, Human, Rabbit, Rat, Sheep more details -
Immunogen
Mouse IgG F(ab)2 fragment
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 4.0 moles FITC per mole of Goat IgG F(ab’)2 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| Flow Cyt |
1/500 - 1/2500.
|
|
| ELISA |
1/10000 - 1/50000.
|
|
| Immunomicroscopy |
Use at an assay dependent dilution.
|
| Notes |
|---|
|
ICC/IF
1/1000 - 1/5000. |
|
Flow Cyt
1/500 - 1/2500. |
|
ELISA
1/10000 - 1/50000. |
|
Immunomicroscopy
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab5883 has not yet been referenced specifically in any publications.
