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Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (HRP), pre-adsorbed (ab98762)

Submit a review Q&A (4)

Key features and details

  • Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (HRP), pre-adsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: WB, ELISA, IHC-P, ICC

Conjugates logo Related conjugates and formulations

Biotin DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC HRP PE TRITC Unconjugated

Overview

  • Product name

    Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (HRP), pre-adsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICCmore details

  • Minimal
    cross-reactivity


    Human, Rat more details

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Horse Radish Peroxidase.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab98762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000 - 1/25000. by chemiluminescent detection. Use at 1/2000 - 1/20000 by colorimetric detection.
ELISA
1/10000 - 1/100000. as primary
IHC-P
1/200 - 1/2000.
ICC
Use at an assay dependent dilution.
Notes
WB
1/5000 - 1/25000. by chemiluminescent detection. Use at 1/2000 - 1/20000 by colorimetric detection.
ELISA
1/10000 - 1/100000. as primary
IHC-P
1/200 - 1/2000.
ICC
Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab98762? Please let us know so that we can cite the reference in this datasheet.

ab98762 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
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1-4 of 4 Abreviews or Q&A

Question

for example this secondary antibody: https://www.abcam.com/Goat-F-ab-2-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fab-2-HRP-pre-adsorbed-ab98762.html
It is a full-length human protein with a C-terminally located Fc-tag.
We want to detect sialic acid (STn) on the O-glycosylation site of the reporter protein by immunoblotting. Blocking is performed with 3% BSA.
Thank you.
Best regards,

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Answer

Thank you very much for the time taken giving me furthrt infotmation.

I can confirm that the Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2 (HRP), pre-adsorbed could overcome the cross-reactivity with the human Fc. However we regrattably cannot give a guarantee for it.

If the immunoblot is performed under denatured and reduced conditions I would recommend to have a look at our anti native antibodies (VeriBlot). This secondary antibodies will not react with denatured proteins. For the use of this antibodies it is improtant to have fully denatured samples. The blocking should be performed with milk (Please read the data sheet carefully). If you https://www.abcam.com/mouse-igg-veriblot-for-ip-secondary-antibody-hrp-ab131368.htmlyou will find the Anti-mouse IgG VeriBlot for IP secondary antibody (HRP) - ab131368 that might be suitable for the experiment.

I hope this information is helpful. If you have further questions please do not hesitate to contact us again.

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Question

vielen Dank für Ihre Informationen!

Nun hat sich aber wieder etwas geändert, denn wir möchten jetzt Anti-Maus-Sekundärantikörper im F(ab)2 Format verwenden, nach wie vor HRP gekoppelt.

Haben Sie da etwas im Angebot, das Epitop sollte nach wie vor die kappa light chain oder das F(ab)2 Fragment sein?





Vielen Dank und beste Grüße

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Answer

Vielen Dank für Ihre Anfrage.
Wir haben jetzt eine Bestätigung von Labor:
Wir haben einen Antikörper, der selbst ein F(ab)2 Fragment ist (gekoppelt an HRP und auch nur F(ab)2 Fragmente bindet:
ab98659 (Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2 (HRP):
Click here (or use the following: https://www.abcam.com/Goat-Fab2-Mouse-IgG-Fab2-HRP-ab98659.html).
Wir haben diesen Antikörper auch "pre-absorbed", was ich Ihnen in diesem Fall auch empfehlen würde:
ab98762 (Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2 (HRP), pre-adsorbed):
Click here (or use the following: https://www.abcam.com/Goat-Fab2-Mouse-IgG-Fab2-HRP-pre-adsorbed-ab98762.html).
I hoffe, diese Information ist hilfreich bei der Auswahl eines anti Maus F(ab)2 Antikörpers für Ihre Experimente und wünsche Ihnen viel Erfolg.

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Question

Thank you so much for your reply. Before I am going to purchase it may I get 5-10ul for test? That would be very helpful.  

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Answer

Thank you for your reply. We do not provide samples of our antibodies but under our Abpromise we do guarantee our products to work as stated on the datasheet. If they fail to perform as stated then we will happily replace or refund the antibody. If there is anything else I can do, please let me kn

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Question

I am looking for anti-mouse IgG heavy chain specific HRP for western blot. The size of my target protein is same with IgG light chain. I need avoid the light chain. I found several products on your website. Is that antibody to mouse IgG-Fc(HRP) can avoid to detect the IgG light chain. What is different with these products? Could you tell me which one is better for my experiment?   ab20043 Goat polyclonal Secondary Antibody to Mouse IgG - Fc (HRP)   ab98762 Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2 (HRP), pre-adsorbed   ab98717 Goat polyclonal Secondary Antibody to Mouse IgG - Fc (HRP), pre-adsorbed   ab98741 Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - Fc (HRP), pre-adsorbed  

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Answer

Thank you for contacting Abcam. Based on your requirements, I would recommend ab20043 as it will only react with the heavy chain of the mouse IgG. The image below shows the different regions of the IgG molecule and so from that you will be able to see clearly the regions that the other antibodies you selected bind too.   The difference between the preabsored and non preabsorbed i antibodies s that preabsorbed antibodies reduce non-specific background staining, especially in IHC when working with closely homologous species. If there is anything else I can help you with, please let me know.  

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