Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (PE), pre-adsorbed (ab5889)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (PE), pre-adsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (PE), pre-adsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: ICC/IF, Immunomicroscopy, Flow Cytmore details -
Minimal
cross-reactivity
Cow, Horse, Human, Rabbit, Rat, Sheep more details -
Immunogen
Mouse IgG F(ab)2 fragment
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Conjugation
PE. Ex: 488nm, Em: 575nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
BSA is IgG and protease free -
Concentration information loading... -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
R-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm OD566nm/OD280nm: 2.6 Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
|
|
| Immunomicroscopy |
Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
|
| Flow Cyt |
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
|
| Notes |
|---|
|
ICC/IF
1/100 - 1/250. |
|
Immunomicroscopy
Use at an assay dependent dilution. Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
|
Flow Cyt
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab5889 has been referenced in 2 publications.
- Shi Q et al. Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cells. Stem Cell Res Ther 8:246 (2017). PubMed: 29096715
- Carocci M et al. Encephalomyocarditis Virus 2A Protein Is Required for Viral Pathogenesis and Inhibition of Apoptosis. J Virol 85:10741-54 (2011). PubMed: 21849462