Goat Anti-Human IgE epsilon chain (ab9159)
Key features and details
- Goat Anti-Human IgE epsilon chain
- Host species: Goat
- Suitable for: ELISA, Immunodiffusion, Immunoelectrophoresis, WB
Overview
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Product name
Goat Anti-Human IgE epsilon chain
See all IgE secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
The affinity purified anti IgE reacts only with IgE ( several different IgE kappa and lambda myeloma proteins) by IEP and gel diffusion techniques. -
Tested applications
Suitable for: ELISA, Immunodiffusion, Immunoelectrophoresis, WBmore details -
Immunogen
Goats were immunized with a mixture of several highly purified human IgE myeloma proteins in Freund’s adjuvant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.194% Methyl mannoside, 0.812% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Serum was collected and passed through several non-IgE containing immunoaffinity supports to make the preparation delta chain specific. The anti delta was immunoaffinity purified off an immunoabsorbant containing a different IgE than was used for immunization thus minimizing idiotypic influences. -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| Immunodiffusion |
Use at an assay dependent concentration.
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| Immunoelectrophoresis |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration.
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| Notes |
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ELISA
Use at an assay dependent concentration. |
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Immunodiffusion
Use at an assay dependent concentration. |
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Immunoelectrophoresis
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (5)
ab9159 has been referenced in 5 publications.
- Malpica L et al. Regulatory T cells and IgE expression in duodenal mucosa of Strongyloides stercoralis and human T lymphotropic virus type 1 co-infected patients. PLoS Negl Trop Dis 13:e0007415 (2019). PubMed: 31170141
- Zhang K et al. Inhibition of Allergic Reactivity through Targeting FceRI-Bound IgE with Humanized Low-Affinity Antibodies. J Immunol 203:2777-2790 (2019). PubMed: 31636239
- Vanshylla K et al. Grb2 and GRAP connect the B cell antigen receptor to Erk MAP kinase activation in human B cells. Sci Rep 8:4244 (2018). PubMed: 29523808
- Zhang K et al. Blocking Allergic Reaction through Targeting Surface-Bound IgE with Low-Affinity Anti-IgE Antibodies. J Immunol 198:3823-3834 (2017). PubMed: 28396318
- Olivier CE et al. In search of a tolerance-induction strategy for cow's milk allergies: significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerization. Clinics (Sao Paulo) 67:1171-9 (2012). PubMed: 23070344
