Goat Anti-Human IgG (Fab')2 (Biotin) preadsorbed (ab64666)
Key features and details
- Goat Anti-Human IgG (Fab')2 (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ELISA, IHC-Fr, Flow Cyt
Overview
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Product name
Goat Anti-Human IgG (Fab')2 (Biotin) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
ab64666 is specific for Fab and F(ab')2 portion of human IgG by IEP. It reacts with other human immunoglobulins through common light chain reactivity. No reactivity with the Fc portion of human IgG or non immunoglobulin serum proteins was detected. -
Tested applications
Suitable for: WB, ELISA, IHC-Fr, Flow Cytmore details -
Minimal
cross-reactivity
Cow, Horse, Mouse more details -
Immunogen
Human IgG F(ab')2
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000.
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ELISA |
1/1000 - 1/10000.
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IHC-Fr |
1/200 - 1/1000.
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|
Flow Cyt |
1/200 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. |
ELISA
1/1000 - 1/10000. |
IHC-Fr
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/1000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab64666 has been referenced in 2 publications.
- Mizukami M et al. Efficient production of Trastuzumab Fab antibody fragments in Brevibacillus choshinensis expression system. Protein Expr Purif 150:109-118 (2018). PubMed: 29857036
- Al-Sajee D et al. Expression of IL-33 and TSLP and Their Receptors in Asthmatic Airways after Inhaled Allergen Challenge. Am J Respir Crit Care Med 198:805-807 (2018). PubMed: 29733677