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Goat Anti-Human IgG (Fab')2 (Biotin) preadsorbed (ab64666)

Submit a review Q&A (1)References (2)

Key features and details

  • Goat Anti-Human IgG (Fab')2 (Biotin) preadsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: WB, ELISA, IHC-Fr, Flow Cyt

Overview

  • Product name

    Goat Anti-Human IgG (Fab')2 (Biotin) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Human

  • Specificity

    ab64666 is specific for Fab and F(ab')2 portion of human IgG by IEP. It reacts with other human immunoglobulins through common light chain reactivity. No reactivity with the Fc portion of human IgG or non immunoglobulin serum proteins was detected.

  • Tested applications

    Suitable for: WB, ELISA, IHC-Fr, Flow Cytmore details

  • Minimal
    cross-reactivity


    Cow, Horse, Mouse more details

  • Immunogen

    Human IgG F(ab')2

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000.
ELISA
1/1000 - 1/10000.
IHC-Fr
1/200 - 1/1000.
Flow Cyt
1/200 - 1/1000.
Notes
WB
1/1000 - 1/10000.
ELISA
1/1000 - 1/10000.
IHC-Fr
1/200 - 1/1000.
Flow Cyt
1/200 - 1/1000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (2)

Publishing research using ab64666? Please let us know so that we can cite the reference in this datasheet.

ab64666 has been referenced in 2 publications.

  • Mizukami M  et al. Efficient production of Trastuzumab Fab antibody fragments in Brevibacillus choshinensis expression system. Protein Expr Purif 150:109-118 (2018). PubMed: 29857036
  • Al-Sajee D  et al. Expression of IL-33 and TSLP and Their Receptors in Asthmatic Airways after Inhaled Allergen Challenge. Am J Respir Crit Care Med 198:805-807 (2018). PubMed: 29733677

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

(1) What is the molecular weight of ab64666?
(2) What does pre-absorbed mean in product ab64666?

Read More
Answer

The average molecular weight of the F(ab')2 fragment is approximately 110 kD.

Preadsorbed means that the antibody was run through a column to reduce cross-reactivity to other species, in this case cow, horse, and mouse.

Read More

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