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Goat Anti-Human IgG Fc (DyLight® 550) preadsorbed (ab98620)

Submit a review Q&A (1)References (1)
Goat Anti-Human IgG Fc (DyLight® 550) preadsorbed (ab98620)

    Key features and details

    • Goat Anti-Human IgG Fc (DyLight® 550) preadsorbed
    • Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: IHC-P, ICC/IF, Flow Cyt

    Conjugates logo Related conjugates and formulations

    Alkaline Phosphatase Biotin DyLight® 488 DyLight® 594 DyLight® 650 FITC HRP SureLight® PE Unconjugated Unconjugated

    Overview

    • Product name

      Goat Anti-Human IgG Fc (DyLight® 550) preadsorbed
      See all IgG secondary antibodies

    • Host species

      Goat

    • Target species

      Human

    • Tested applications

      Suitable for: IHC-P, ICC/IF, Flow Cytmore details

    • Minimal
      cross-reactivity


      Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, Sheep more details

    • Conjugation

      DyLight® 550. Ex: 562nm, Em: 576nm

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituents: PBS, 0.2% BSA
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      ab98620 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • General notes

      DyLight® 550 replaces DyLight® 549.

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab98620 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/50 - 1/500.
    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.
    Notes
    IHC-P
    1/50 - 1/500.
    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.

    Images

    • Goat Anti-Human IgG Fc (DyLight® 550) preadsorbed (ab98620)
      Goat Anti-Human IgG Fc (DyLight® 550) preadsorbed (ab98620)
      Emission spectra of DyLight® fluorochromes available in our catalog.
      Line colors represent the approximate visible colors of the wavelength maxima.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab98620? Please let us know so that we can cite the reference in this datasheet.

    ab98620 has been referenced in 1 publication.

    • Mathias S  et al. Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy. J Biotechnol 271:47-55 (2018). ICC . PubMed: 29477386

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Dir Sir or Madame,




    I am looking for an anti human IgG1 antibody, which detects the human Fc (IgG1: Pro 100- Lys 300) in a FACS stainig without cross reactivity to murine IgG (CH2 CH3)

    The type of fluorophores does not matter.



    Kind regards,

    Read More
    Answer

    Thank you for your inquiry.

    I can confirm that we do not have anti human IgG1 antibodies.

    But Iam very happy to confirm thatwe do have anti human (pan) IgG in four colors that are pre-absorbed against mice and show only minimal cross reaction.

    Please follow the links to review the datasheets:

    ab98564

    https://www.abcam.com/Goat-Human-IgG-Fc-DyLight-594-preadsorbed-ab98564.html (or use the following: https://www.abcam.com/Goat-Human-IgG-Fc-DyLight-594-preadsorbed-ab98564.html).

    ab98565

    https://www.abcam.com/Goat-Human-IgG-Fc-DyLight-650-preadsorbed-ab98565.html (or use the following: https://www.abcam.com/Goat-Human-IgG-Fc-DyLight-650-preadsorbed-ab98565.html).

    ab98619

    https://www.abcam.com/Goat-Human-IgG-Fc-DyLight-488-preadsorbed-ab98619.html (or use the following: https://www.abcam.com/Goat-Human-IgG-Fc-DyLight-488-preadsorbed-ab98619.html).

    ab98620

    https://www.abcam.com/Goat-Human-IgG-Fc-DyLight-550-preadsorbed-ab98620.html (or use the following: https://www.abcam.com/Goat-Human-IgG-Fc-DyLight-550-preadsorbed-ab98620.html).

    I hope that one of these antibodies will be the right choice for your experiments. Please feel free to contact me again with any further questions in this matter.

    Read More

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