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Goat Anti-Human IgG H&L (10nm Gold) (ab39596)

Submit a review Q&A (1)References (1)

Key features and details

  • Goat Anti-Human IgG H&L (10nm Gold)
  • Conjugation: Gold 10nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: Electron Microscopy

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Overview

  • Product name

    Goat Anti-Human IgG H&L (10nm Gold)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Human

  • Tested applications

    Suitable for: Electron Microscopymore details

  • Immunogen

    Human IgG whole molecule.

  • Conjugation

    Gold 10nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 10nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 10nm particle size conjugate is less than 10 %.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab39596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
Notes
Application notes
Electron Microscopy: Use at an assay dependent dilution.
Light Microscopy: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab39596? Please let us know so that we can cite the reference in this datasheet.

ab39596 has been referenced in 1 publication.

  • Rafat N  et al. Enhanced Enzymatically Amplified Metallization on Nanostructured Surfaces for Multiplexed Point-of-Care Electrical Detection of COVID-19 Biomarkers. Small 18:e2203309 (2022). PubMed: 36036173

Customer reviews and Q&As

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Question



Regarding the Goat Anti-Human IgG H&L (6 or 10 nm Gold) [ab39596 and ab39618​] I would like to know the concentration of the gold particles and how many IgGs are bound per particle. How are the IgGs coupled to the gold?

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Answer



1. Concentration of the gold particles

Goat Anti-Human IgG H&L (6nm Gold): 2.4·10^13 particles per ml

Goat Anti-Human IgG H&L (10nm Gold): 5·10^12 particles per ml


2. How many IgGs are bound per particle

Goat Anti-Human IgG H&L (6nm Gold): 1 - 2

Goat Anti-Human IgG H&L (10nm Gold): 7- 12


3. How are the IgGs coupled to the gold?

This is an adsorption/chemisorption technique. The conventional labeling approach in transmission and scanning electron microscopy utilizes secondary immunogold reagents based on particles that can be observed without enhancement. These conjugates are suited for single and multiple labeling in electron microscopy, when the number of antigens available for binding is such that a relevant signal can be obtained.

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