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    products/secondary-antibodies/goat-human-igg-hl-cy5--preadsorbed-ab97172.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Human IgG H&L (Cy5 ®) preadsorbed (ab97172)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

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Promotion Information

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Key features and details

  • Goat Anti-Human IgG H&L (Cy5 ®) preadsorbed
  • Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ICC/IF, Flow Cyt

Conjugates logo Related conjugates and formulations

Alkaline Phosphatase Biotin Cy3 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 6nm HRP Texas Red ® TRITC Unconjugated

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Overview

  • Product name

    Goat Anti-Human IgG H&L (Cy5 ®) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Human
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, mouse, pig, rabbit and rat was detected.

     

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Horse, Mouse, Pig, Rabbit, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Other Immunogen Type corresponding to Human IgG.

  • Conjugation

    Cy5 ®. Ex: 650nm, Em: 667nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, horse, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy5 ®.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Human
    • IgG
    • Fluorophore
    • Cy5

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50 - 1/500.
ICC/IF
1/50 - 1/500.
Flow Cyt
1/100 - 1/1000.
Notes
IHC-P
1/50 - 1/500.
ICC/IF
1/50 - 1/500.
Flow Cyt
1/100 - 1/1000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab97172? Please let us know so that we can cite the reference in this datasheet.

ab97172 has been referenced in 2 publications.

  • Kaneyoshi K  et al. Analysis of the immunoglobulin G (IgG) secretion efficiency in recombinant Chinese hamster ovary (CHO) cells by using Citrine-fusion IgG. Cytotechnology 71:193-207 (2019). PubMed: 30610509
  • Valentini D  et al. Peptide microarray-based characterization of antibody responses to host proteins after bacille Calmette-Guérin vaccination. Int J Infect Dis 56:140-154 (2017). PubMed: 28161459

Customer reviews and Q&As

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