Goat Anti-Human IgG H&L (FITC) (ab6854)
Key features and details
- Goat Anti-Human IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA
Related conjugates and formulations
Overview
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Product name
Goat Anti-Human IgG H&L (FITC)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Tested applications
Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details -
Immunogen
human whole molecule
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.164% Sodium phosphate, 0.878% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunomicroscopy |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent dilution.
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|
| ICC/IF |
1/1000 - 1/5000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| ELISA |
1/10000 - 1/50000.
|
| Notes |
|---|
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent dilution. |
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab6854 has been referenced in 5 publications.
- Zhou C et al. Cancer-associated adipocytes promote the invasion and metastasis in breast cancer through LIF/CXCLs positive feedback loop. Int J Biol Sci 18:1363-1380 (2022). PubMed: 35280694
- Bao L et al. Susceptibility and Attenuated Transmissibility of SARS-CoV-2 in Domestic Cats. J Infect Dis 223:1313-1321 (2021). PubMed: 33605423
- Wang C et al. Synergistic antitumor effect of the anti-ErbB2 antibodies trastuzumab and H2-18 on trastuzumab-resistant gastric cancer cells. Oncol Lett 21:400 (2021). PubMed: 33777223
- Wangzhou K et al. MiR-143-3p Inhibits Osteogenic Differentiation of Human Periodontal Ligament Cells by Targeting KLF5 and Inactivating the Wnt/ß-Catenin Pathway. Front Physiol 11:606967 (2020). PubMed: 33603676
- Farrera-Soler L et al. Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma. PLoS One 15:e0238089 (2020). PubMed: 32903266