Goat Anti-Human IgG H&L preadsorbed (ab7148)
Key features and details
- Goat Anti-Human IgG H&L preadsorbed
- Host species: Goat
- Suitable for: WB, IHC-P, IHC-Fr, IP, ICC/IF, Immunodiffusion, Conjugation, ELISA
Overview
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Product name
Goat Anti-Human IgG H&L preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, IP, ICC/IF, Immunodiffusion, Conjugation, ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep more details -
Immunogen
Human IgG whole molecule
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
|
| Immunodiffusion |
Use at an assay dependent concentration.
|
|
| Conjugation |
Use at an assay dependent concentration.
|
|
| ELISA |
1/25000 - 1/120000.
|
| Notes |
|---|
|
WB
1/2000 - 1/10000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
ICC/IF
1/1000 - 1/5000. |
|
Immunodiffusion
Use at an assay dependent concentration. |
|
Conjugation
Use at an assay dependent concentration. |
|
ELISA
1/25000 - 1/120000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab7148 has been referenced in 1 publication.
- Pinto IF et al. Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production. ACS Sens 6:842-851 (2021). PubMed: 33724791