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Goat Anti-Mouse IgG Fc (Biotin) preadsorbed (ab98711)

Submit a review Q&A (1)References (1)

Key features and details

  • Goat Anti-Mouse IgG Fc (Biotin) preadsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: WB, ELISA, ICC/IF, IHC-P

Overview

  • Product name

    Goat Anti-Mouse IgG Fc (Biotin) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: WB, ELISA, ICC/IF, IHC-Pmore details

  • Minimal
    cross-reactivity


    Human, Rat more details

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab98711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/200000. (Chemiluminescent detection).
ELISA
1/10000 - 1/200000. (as Primary).
ICC/IF
1/100 - 1/500.
IHC-P
1/250 - 1/2500.
Notes
WB
1/10000 - 1/200000. (Chemiluminescent detection).
ELISA
1/10000 - 1/200000. (as Primary).
ICC/IF
1/100 - 1/500.
IHC-P
1/250 - 1/2500.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab98711? Please let us know so that we can cite the reference in this datasheet.

ab98711 has been referenced in 1 publication.

  • Lee JR  et al. Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus. Sci Rep 6:27623 (2016). PubMed: 27279139

Customer reviews and Q&As

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Question

Hi,



I have some antibodies from you, and their datasheets say that I need to store them at 4C, for example, ab98711 and ab692.

However, most of antibodies from abcam need to be stored at –20C or –80C.



So, my question is what is the estimated shelf life of the product if I store it at 4C as its datasheet says.

I’m worried whether it is correct that they are loosing their affinity or denatured with a higher rate compared to other antibodies kept at –20C as time goes by.
And, what happens if I freeze it?

Thank you in advance.

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Answer

Thank you for your enquiry.

Estimated shelf life of antibodies stored at 4 degrees C is about 1 year, although frequently they last much longer.

I recommend that you follow the storage conditions as noted on the product datasheet. Freezing an antibody that is intended to only be stored at 4 degrees C may lead to denaturing of the antibody.

I hope this is helpful. Please contact me again if you have any further questions.

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