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Goat Anti-Mouse IgG Fc (HRP) (ab97265)

Submit a review Q&A (9)References (34)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG Fc (HRP) (ab97265)

    Key features and details

    • Goat Anti-Mouse IgG Fc (HRP)
    • Conjugation: HRP
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: ICC, IHC-P, ELISA, WB

    Conjugates logo Related conjugates and formulations

    Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 12nm Gold 6nm Unconjugated Unconjugated

    Overview

    • Product name

      Goat Anti-Mouse IgG Fc (HRP)
      See all IgG secondary antibodies

    • Host species

      Goat

    • Target species

      Mouse

    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG-Fc fagment. Cross reactivity with IgA and IgM is negligible. This antibody may cross react with IgG from other species.

    • Tested applications

      Suitable for: ICC, IHC-P, ELISA, WBmore details

    • Conjugation

      HRP

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 6.8
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

    • Conjugation notes

      Molar enzyme/ antibody protein ratio is 4:1

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab97265 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC
    Use at an assay dependent dilution.
    IHC-P
    1/200 - 1/2000.
    ELISA
    1/10000 - 1/100000. (Primary).
    WB
    1/2000 - 1/25000. Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000
    Notes
    ICC
    Use at an assay dependent dilution.
    IHC-P
    1/200 - 1/2000.
    ELISA
    1/10000 - 1/100000. (Primary).
    WB
    1/2000 - 1/25000. Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG Fc (HRP) (ab97265)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG Fc (HRP) (ab97265)

      IHC image of beta actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97265,  1/1000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

      The inset negative control image is secondary-only at 1/200 dilution.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (34)

    Publishing research using ab97265? Please let us know so that we can cite the reference in this datasheet.

    ab97265 has been referenced in 34 publications.

    • Koretz KS  et al. Characterization of binding kinetics of A2AR to Gas protein by surface plasmon resonance. Biophys J 120:1641-1649 (2021). PubMed: 33675761
    • Herrero E  et al. Asymmetric Transcription Factor Partitioning During Yeast Cell Division Requires the FACT Chromatin Remodeler and Cell Cycle Progression. Genetics 216:701-716 (2020). PubMed: 32878900
    • Gamella M  et al. First electrochemical immunosensor for the rapid detection of mustard seeds in plant food extracts. Talanta 219:121247 (2020). PubMed: 32887138
    • Streletskaia AY  et al. Upregulation of Mcl-1S Causes Cell-Cycle Perturbations and DNA Damage Accumulation. Front Cell Dev Biol 8:543066 (2020). PubMed: 33072738
    • Jain AR  et al. The Specificity of Downstream Signaling for A1 and A2AR Does Not Depend on the C-Terminus, Despite the Importance of This Domain in Downstream Signaling Strength. Biomedicines 8:N/A (2020). PubMed: 33322210
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-9 of 9 Abreviews or Q&A

    Question


    Inquiry: Hi Abcam, I have had great success using this secondary Ab for westerns that involve a IgG1 primary with a film exposure time of only 15s-30s. Just recently I ran a western of a recombinant protein, detecting with an IgG2a primary Ab with this as the secondary and the band took about 5 mins to develop. I know that this is not a lack of protein because I an loading a large amount of the recombinant. I also know that the primary Ab will bind this protein from earlier work. Have there been other issues reported for using this secondary with a IgG2a primary? Thanks in advance.

    Read More
    Answer

    Thank you for contacting us.


    This antibody should work for all IgG subclasses. Although you know your primary works, perhaps it is somewhat less efficient than the primary antibody with the IgG2a subclass?

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Hello, Will this product react with all mouse IgG Fc's including mouse IgG1 Fc? Thanks

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    Answer

    Thank you for contacting us.

    Yes, this antibody will react with the Fc domain of all mouse IgG including IgG1, but not IgA or IgM.

    Read More

    Question

    Could I have certificate of analysis of following products please:
    Product
    Référence
    N°lot
    Mouse monoconal to Human Serum Albumin
    ab10241
    3002109900
    Mouse monoclonal to Transferrin
    ab70826
    3002109900
    Goat polyclonal Secondary Antibody to Mouse IgG-Fc (HRP)
    ab97265
    3002109900
    Best regards

    Read More
    Answer

    Thank you for contacting us.

    Please find attached certificate of compliance. We do not provide certificate of analysis for antibodies.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Question

    Product code: 97265
    Lot number: GR78227-1

    Inquiry: Hi, Could you send me the certificate of compliance of the product above.

    Read More
    Answer

    Merci de nous avoir contactés.
    Veuillez trouver ci-joint le certificat dont vous avez fait la demande.
    J'espère que ce document vous sera utile. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Question



    wir wären an diesem Angebot sehr interessiert und würden gerne diesen Antikörper testen.

    Ich habe letzte Woche schon eine Email an Ihre secondary antibodies product manager geschickt, allerdings noch keine Rückmeldung bezüglich eines Gutscheincodes erhalten.

    Ich würde mich freuen, von Ihnen zu hören.

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    Answer



    Wenn Sie in Zukunft ein Produkt testen möchten, oder allgemin mehr über einen Antikörper oder eines unserer Produkte wissen möchten, wenden Sie sich an uns vom technischen Dienst (wissenschaftlicherdienst@abcam.com). Frau Langenick arbeitet in der Marketingabteilung und leitet Ihre Anfragen zu uns weiter, und da kann es manchmal dauern, bis wir Ihre Anfrage dann bearbeiten können. Allerdings ist secondary antibodies product manager wiederum dafür der richtige Ansprechpartner für Ihre Ergebnisse (obwohl wir die auch bearbeiten können). Ich hoffe, dies ist jetzt nicht zu verwirrend.



    DISCOUNT CODE: XXXXXX
    Ablaufdatum: YYYYYYYY


    Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab97265 Goat anti-Mouse IgG Fc (HRP) secondary antibody in In-Cell-ELISA (ICE) zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer XXXXXX, deckt den Wert von ab97265 ab, und kann zum Kauf weiterer Produkte eingesetzt werden.

    Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in (ICE) eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

    Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

    Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

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    Question

    Dear All,



    Sending you attached a list of the tendered items. You can see the products at page 3-5. Please give all of the items a check and let us know which items are best available with Abcam.



    Early response requested.



    Sincere regards,

    Read More
    Answer

    Thank you for your inquiry.

    We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

    Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

    Please find a list of the product code and the links to the respective datasheets below:

    ab1012 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade)

    https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-mAbcam1012-ChIP-Grade-ab1012.html (or use the following: https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-mAbcam1012-ChIP-Grade-ab1012.html).

    ab12209 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade)

    https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-mAbcam12209-ChIP-Grade-ab12209.html (or use the following: https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-mAbcam12209-ChIP-Grade-ab12209.html).

    ab6002 (Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade)

    https://www.abcam.com/Histone-H3-tri-methyl-K27-antibody-mAbcam-6002-ChIP-Grade-ab6002.html (or use the following: https://www.abcam.com/Histone-H3-tri-methyl-K27-antibody-mAbcam-6002-ChIP-Grade-ab6002.html).

    ab124462 (Anti-Flag Tag antibody)

    https://www.abcam.com/DDDDK-tag-antibody-Equivalent-to-FLAG-antibodies-from-Sigma-ab124462.html (or use the following: https://www.abcam.com/DDDDK-tag-antibody-Equivalent-to-FLAG-antibodies-from-Sigma-ab124462.html).

    ab6939 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed)

    https://www.abcam.com/Goat-Rabbit-IgG-HL-Cy3--preadsorbed-ab6939.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-Cy3--preadsorbed-ab6939.html).

    ab96899 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed)

    https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96899.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96899.html).

    ab96919 (Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody)

    https://www.abcam.com/Donkey-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96919.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96919.html).

    ab96879 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed)

    https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-preadsorbed-ab96879.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-preadsorbed-ab96879.html).

    ab96871 (Goat anti-Mouse IgG H&L (DyLight® 488) secondary antibody)

    https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-ab96871.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-ab96871.html).

    ab97265 (Goat anti-Mouse IgG Fc (HRP) secondary antibody)

    https://www.abcam.com/Goat-Mouse-IgG-Fc-HRP-ab97265.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG-Fc-HRP-ab97265.html).

    ab7481 (Goat Serum)

    https://www.abcam.com/Normal-Goat-Serum-ab7481.html (or use the following: https://www.abcam.com/Normal-Goat-Serum-ab7481.html).

    ab7487 (Rabbit Serum (sterile))

    https://www.abcam.com/Normal-Rabbit-Serum-Sterile-ab7487.html (or use the following: https://www.abcam.com/Normal-Rabbit-Serum-Sterile-ab7487.html).

    ab46666 (Herring sperm ssDNA)

    https://www.abcam.com/Herring-sperm-ssDNA-ab46666.html (or use the following: https://www.abcam.com/Herring-sperm-ssDNA-ab46666.html).

    ab52579 (Ribonuclease A protein (Active))

    https://www.abcam.com/Natural-cow-Ribonuclease-A-protein-ab52579.html (or use the following: https://www.abcam.com/Natural-cow-Ribonuclease-A-protein-ab52579.html).

    ab73430 (DNase I protein (Active))

    https://www.abcam.com/Recombinant-human-DNase-I-protein-Active-ab73430.html (or use the following: https://www.abcam.com/Recombinant-human-DNase-I-protein-Active-ab73430.html).

    ab64220 (Proteinase K)

    https://www.abcam.com/Proteinase-K-Antigen-Retrieval-Solution-ab64220.html (or use the following: https://www.abcam.com/Proteinase-K-Antigen-Retrieval-Solution-ab64220.html).

    ab65621 (Protease Inhibitor Cocktail)

    https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html (or use the following: https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html).

    ab116028 (Prism Ultra Protein Ladder (10-245 kDa))

    https://www.abcam.com/Prestained-Protein-Ladder-Broad-molecular-weight-10-245-kDa-ab116028.html (or use the following: https://www.abcam.com/Prestained-Protein-Ladder-Broad-molecular-weight-10-245-kDa-ab116028.html).


    I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

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    Question

    We have bought the following conjugates from your company. We asked the agancy that we bought about the temperature to keep them to increase the time of keeping. They told us to keep them in -20 C0 and we did for two weeks up to now, but now when we checked your website information it is recommended to be kept in 4 degree castigates.

    Please let me know if keeping them in -20 C0 has made any problem for them and can we continue to keep them in this temperature or not?

    Goat-polyclonal-Secondary-Antibody-to-Human-IgG-Fc-HRP(ab97225)

    Goat-polyclonal-Secondary-Antibody-to-Bird-IgG-H-L-HRP(ab112773)

    Goat-polyclonal-Secondary-Antibody-to-Horse-IgG-H-L-HRP(ab102396)

    Goat-polyclonal-Secondary-Antibody-to-Syrian-Hamster-IgG-H-L(HRP-ab6892)

    Goat-polyclonal-Secondary-Antibody-to-Human-IgM-mu-chain-HRP(ab97205)

    Goat-polyclonal-Secondary-Antibody-to-Cow-IgG-H-L-HRP(ab6934)

    Rabbit-polyclonal-Secondary-Antibody-to-Sheep-IgG-Fc-HRP (ab102299)

    Rabbit-polyclonal-Secondary-Antibody-to-Goat-IgG-Fc-HRP-pre-adsorbed (ab102345)

    Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fc-HRP (ab97265)

    Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-Fc-HRP-pre-adsorbed (ab97090)


    Best,

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    Answer

    Thank you for contacting us.

    The datasheet of these antibodies say "Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles"

    This mean after receiving the antibody you can keep antibody at 4C for 1-2 weeks when using in your experiments. After you finish your experiment we recomendaliquoting the antibody 5-10 ul in vialsand storing at -20C. We suggest this to avoidthe extra freeze thawing steps, every time you use the antibody.

    If you have already stored it at -20C, this is absolutely fine!

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Please let us know regarding the proteins or strain which have been used for below products as desired by one of our customer : ab106292 ab34749 ab108750 ab97265  ( Which H+ or which chain ( Light) ab108750 ( Referance for the same)

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    Answer

    Thank you for contacting us.  I am still waiting for additional information regarding the strain used to generate ab106292, but I will let you know as soon as I hear back from the lab regarding this.  The other products are as follows: ab34749: 9B11 was generated against full-length recombinant Rubella capsid protein expressed in yeast S.cerevisiae. To produce the recombinant antigen, RNA was isolated from Rubella virus strain RA27/3 (GenBank: X14871.1). ab97265: This secondary antibody is specific to the Fc region of Mouse IgG and shows limited cross-reactivity with IgM and IgA. ab108750: The plates used in the kit are coated with Edmonston strain of the measles virus.  I hope this helps, please let me know if you need any additional information. 

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    Question

    Do you know what the sequence is that the antibody detects on the IgG Fc part? (Goat anti mouse ab97265)

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    Answer

    Thank you for contacting us. Unfortunately I am not able to provide information about the exact antibody binding sequence for ab97265 as this is considered proprietary by the lab that generated the product. As stated on the product specificity, this antibody reacts specifically with Mouse IgG-Fc. Sorry I could not be of more help in this instance. Please do not hesitate to contact us should you have further questions.

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