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Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)

Submit a review Q&A (4)References (1)

Key features and details

  • Goat Anti-Mouse IgG Fc (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ELISA, WB, ICC

Overview

  • Product name

    Goat Anti-Mouse IgG Fc (HRP) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species.

  • Tested applications

    Suitable for: IHC-P, ELISA, WB, ICCmore details

  • Minimal
    cross-reactivity


    Human, Rat more details

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab98717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/200 - 1/2000.
ELISA
1/10000 - 1/100000. (Primary).
WB
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000).
ICC
Use at an assay dependent dilution.
Notes
IHC-P
1/200 - 1/2000.
ELISA
1/10000 - 1/100000. (Primary).
WB
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000).
ICC
Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab98717? Please let us know so that we can cite the reference in this datasheet.

ab98717 has been referenced in 1 publication.

  • Behzadi MA  et al. A cross-reactive mouse monoclonal antibody against rhinovirus mediates phagocytosis in vitro. Sci Rep 10:9750 (2020). PubMed: 32546721

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Question

I am looking for a HRP conjugated anti-mouse antibody to be used in ELISA. The most important thing is that it will have the least cross reactivity to rat immunoglobulins. I see that you have many such antibodies. Which one would you think is the most suitable?


Thanks,

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Answer

Thank you for contacting us.

The following products would be suitable as per your requirements. The catalogue numbers are

ab5870, ab5887, ab7061, ab7068, ab98717, ab98790 etc

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Thank you so much for your reply. Before I am going to purchase it may I get 5-10ul for test? That would be very helpful.  

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Answer

Thank you for your reply. We do not provide samples of our antibodies but under our Abpromise we do guarantee our products to work as stated on the datasheet. If they fail to perform as stated then we will happily replace or refund the antibody. If there is anything else I can do, please let me kn

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Question

I am looking for anti-mouse IgG heavy chain specific HRP for western blot. The size of my target protein is same with IgG light chain. I need avoid the light chain. I found several products on your website. Is that antibody to mouse IgG-Fc(HRP) can avoid to detect the IgG light chain. What is different with these products? Could you tell me which one is better for my experiment?   ab20043 Goat polyclonal Secondary Antibody to Mouse IgG - Fc (HRP)   ab98762 Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2 (HRP), pre-adsorbed   ab98717 Goat polyclonal Secondary Antibody to Mouse IgG - Fc (HRP), pre-adsorbed   ab98741 Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - Fc (HRP), pre-adsorbed  

Read More
Answer

Thank you for contacting Abcam. Based on your requirements, I would recommend ab20043 as it will only react with the heavy chain of the mouse IgG. The image below shows the different regions of the IgG molecule and so from that you will be able to see clearly the regions that the other antibodies you selected bind too.   The difference between the preabsored and non preabsorbed i antibodies s that preabsorbed antibodies reduce non-specific background staining, especially in IHC when working with closely homologous species. If there is anything else I can help you with, please let me know.  

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Question

Dear order team,   We received empty vial of ab98717 and the cap was opened.   lot# GR56315-1 order no 988136 invoice no 103411 invoice date 25 Nov   Please arrange a replacement for our customer.   Thank you in advance.     Best regards,

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Answer

This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial of ab98717 as a free of charge replacement. I hope the second vial will work as it is expected, and please do let me know how you are getting on with this product.

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