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Goat Anti-Mouse IgG H&L (ab6708)

Submit a review Submit a question References (49)
Western blot - Goat Anti-Mouse IgG H&L (ab6708)
  • Western blot - Goat Anti-Mouse IgG H&L (ab6708)

Key features and details

  • Goat Anti-Mouse IgG H&L
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WB

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WBmore details

  • Immunogen

    Mouse IgG, whole molecule

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/150000.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
1/3000 - 1/15000.
Notes
ELISA
1/150000.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
1/3000 - 1/15000.

Images

  • Western blot - Goat Anti-Mouse IgG H&L (ab6708)
    Western blot - Goat Anti-Mouse IgG H&L (ab6708)
    Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution + Mouse IgG at 0.1 µg

    Secondary
    Secondary antibody: DyLight™ 800 goat secondary antibody at 1/20000 dilution

    Observed band size: 28,55 kDa why is the actual band size different from the predicted?



    ab6708 incubation overnight at 4°C.

    Both heavy (55kDa) and light (28kDa) chains are detected.

  • Western blot - Goat Anti-Mouse IgG H&L (ab6708)
    Western blot - Goat Anti-Mouse IgG H&L (ab6708)
    All lanes : Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution

    Lane 1 : Mouse IgG (whole molecule)
    Lane 2 : Mouse IgG Fc fragment
    Lane 3 : Mouse IgG Fab fragment
    Lane 4 : Mouse IgM kappa
    Lane 5 : Mouse serum

    Lysates/proteins at 0.05 µg per lane.

    Secondary
    All lanes : HRP Donkey Anti-Goat IgG at 1/40000 dilution


    ab6708 incubated for 1 hour at room temperature.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (49)

Publishing research using ab6708? Please let us know so that we can cite the reference in this datasheet.

ab6708 has been referenced in 49 publications.

  • Kim SH  et al. GlycoGrip: Cell Surface-Inspired Universal Sensor for Betacoronaviruses. ACS Cent Sci 8:22-42 (2022). PubMed: 35106370
  • Dong Z  et al. Increased expression of SRPK1 (serine/arginine-rich protein-specific kinase 1) is associated with progression and unfavorable prognosis in cervical squamous cell carcinoma. Bioengineered 13:6100-6112 (2022). PubMed: 35192432
  • Maywald ML  et al. Rap1 Activity Is Essential for Focal Adhesion and Slit Diaphragm Integrity. Front Cell Dev Biol 10:790365 (2022). PubMed: 35372328
  • Huang Y  et al. Parkinson's Disease Derived Exosomes Aggravate Neuropathology in SNCA*A53T Mice. Ann Neurol 92:230-245 (2022). PubMed: 35596947
  • Schürmann H  et al. Analysis of monocyte cell tractions in 2.5D reveals mesoscale mechanics of podosomes during substrate-indenting cell protrusion. J Cell Sci 135:N/A (2022). PubMed: 35621127
View all Publications for this product

Customer reviews and Q&As

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