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    products/secondary-antibodies/goat-mouse-igg-hl-alexa-fluor-405-ab175660.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (9)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
  • Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)

Key features and details

  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 405)
  • Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 10nm Gold 6nm HRP HRP polymer IRDye® 680RD PE Texas Red ® TRITC Unconjugated Unconjugated

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L (Alexa Fluor® 405)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 405. Ex: 402nm, Em: 421nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Fluorophore
    • Alexa Fluor® 405

Associated products

  • Alternative Versions

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) (ab150114)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 568) (ab175473)
    • Goat Anti-Mouse IgG H&L (ab182017)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (Biotin) (ab207996)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.

We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) 
Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm 
Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm

Flow Cyt
1/2000.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.

We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) 
Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm 
Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm

Flow Cyt
1/2000.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)

    ICC/IF image of ab7291 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (blue) was ab175660 Alexa Fluor® 405 goat anti-mouse IgG (H+L) used at 1µg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
    ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)

    Cross-reactivity of the polyclonal secondary antibody ab182017 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182017 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.

    Fot the batch tested, ab182017 showed a cross-reactivity below 2% towards Chicken IgY, 6% towards Human IgG, 7% towards Rabbit IgG and 47% towards Rat IgG.

    This data was developed using the unconjugated antibody (ab182017).

  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
    ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)

    Cross-reactivity of Goat anti-Mouse IgG H&L (ab182017) and Goat anti-Mouse IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

    This data was developed using the unconjugated antibody (ab182017).

  • Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
    Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (9)

Publishing research using ab175660? Please let us know so that we can cite the reference in this datasheet.

ab175660 has been referenced in 9 publications.

  • Nakagawa Y  et al. Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive Inhibition. Cell Mol Gastroenterol Hepatol 11:949-971 (2021). PubMed: 33246135
  • Milan PB  et al. Copper-enriched diamond-like carbon coatings promote regeneration at the bone-implant interface. Heliyon 6:e03798 (2020). PubMed: 32368647
  • Cabukusta B  et al. Human VAPome Analysis Reveals MOSPD1 and MOSPD3 as Membrane Contact Site Proteins Interacting with FFAT-Related FFNT Motifs. Cell Rep 33:108475 (2020). PubMed: 33296653
  • Hanafusa K & Hayashi N The Flot2 component of the lipid raft changes localization during neural differentiation of P19C6 cells. BMC Mol Cell Biol 20:38 (2019). PubMed: 31455216
  • Lobo CIV  et al. Dual-species biofilms of Streptococcus mutans and Candida albicans exhibit more biomass and are mutually beneficial compared with single-species biofilms. J Oral Microbiol 11:1581520 (2019). PubMed: 31681463
  • Saint-Martin M  et al. Impact of anti-CASPR2 autoantibodies from patients with autoimmune encephalitis on CASPR2/TAG-1 interaction and Kv1 expression. J Autoimmun 103:102284 (2019). PubMed: 31176559
  • Yamada M  et al. Muscle-derived SDF-1a/CXCL12 modulates endothelial cell proliferation but not exercise training-induced angiogenesis. Am J Physiol Regul Integr Comp Physiol 317:R770-R779 (2019). PubMed: 31577158
  • Bernkopf DB  et al. Pgam5 released from damaged mitochondria induces mitochondrial biogenesis via Wnt signaling. J Cell Biol 217:1383-1394 (2018). PubMed: 29438981
  • Wu X  et al. Folic acid reverses uric acid crystal-induced surface OAT1 internalization by inhibiting RhoA activity in uric acid nephropathy. Mol Med Rep 13:2385-92 (2016). PubMed: 26846716

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Triple staining of Purkinje cell with NCX1 and granule cells.

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA fixed)
Confocal image of Purkinje cell (red) in cerebellum with NCX1 (green) and granule cells (blue). The cerebellar slice cultures of mouse cerebellum were fixed with 4% paraformaldehyde(6 hours at 4°C) and permeabilized with 0.5% Triton X-100. Cerebellar slices were incubated with primary antibodies (Calbindin-1:500, NCX1-1:100, and NeuN-1: 500 in PB + 3% NGS + 0.5% Triton X-100) at 4°C overnight. The secondary antibody ab175660 Alexa Fluor® 405 goat anti-mouse IgG (H+L), 1:500 was used to stain the granule cells (blue) together with other secondary antibodies Alexa 568 goat anti guinea pig for Purkinje cell (red) and Alexa 488 goat anti rabbit for NCX1 (green) at ambient temperature for 2 hours.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Pradeep Sherkhane

Verified customer

Submitted May 02 2017

Question

Could you please tell me if Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660) is exactly the same as A-31553 from Life Technology.

Best

Read More

Abcam community

Verified customer

Asked on Dec 05 2013

Answer


ab175660 is extensively tested complete Abcam antibody i.e. it is developed in Abcam laboratory.

Please note that the Alexa Fluor conjugates are patented by life technologies so Abcam use the same conjugate under sale license.

Read More

Padamjeet Singh

Abcam Scientific Support

Answered on Dec 05 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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