Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
Key features and details
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 405)
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA
Related conjugates and formulations
Overview
-
Product name
Goat Anti-Mouse IgG H&L (Alexa Fluor® 405)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details -
Immunogen
The details of the immunogen for this antibody are not available.
-
Conjugation
Alexa Fluor® 405. Ex: 402nm, Em: 421nm
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
-
Research areas
Associated products
-
Alternative Versions
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) (ab150114)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 568) (ab175473)
- Goat Anti-Mouse IgG H&L (ab182017)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
- Goat Anti-Mouse IgG H&L (Biotin) (ab207996)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/200 - 1/1000.
We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
|
| Flow Cyt |
1/2000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ELISA |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
1/200 - 1/1000. We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
|
Flow Cyt
1/2000. |
|
IHC-P
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
Images
-
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
ICC/IF image of ab7291 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (blue) was ab175660 Alexa Fluor® 405 goat anti-mouse IgG (H+L) used at 1µg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
-
ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)Cross-reactivity of the polyclonal secondary antibody ab182017 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182017 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.
Fot the batch tested, ab182017 showed a cross-reactivity below 2% towards Chicken IgY, 6% towards Human IgG, 7% towards Rabbit IgG and 47% towards Rat IgG.
This data was developed using the unconjugated antibody (ab182017).
-
ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)Cross-reactivity of Goat anti-Mouse IgG H&L (ab182017) and Goat anti-Mouse IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.
This data was developed using the unconjugated antibody (ab182017).
-
Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (17)
ab175660 has been referenced in 17 publications.
- Yoon S et al. Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes. Cell Death Differ 29:306-322 (2022). PubMed: 34999730
- Pal K et al. Single-Molecule Force Imaging Reveals That Podosome Formation Requires No Extracellular Integrin-Ligand Tensions or Interactions. ACS Nano 16:2481-2493 (2022). PubMed: 35073043
- Wang X et al. Up-regulation of cell division cycle 20 expression alters the morphology of neuronal dendritic spines in the nucleus accumbens by promoting FMRP ubiquitination. J Neurochem 162:166-189 (2022). PubMed: 35621027
- Nakagawa Y et al. Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive Inhibition. Cell Mol Gastroenterol Hepatol 11:949-971 (2021). PubMed: 33246135
- Ma H et al. Amygdala-hippocampal innervation modulates stress-induced depressive-like behaviors through AMPA receptors. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33526688