Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020)
Key features and details
- Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, ELISA, WB, IHC-P
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Overview
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Product name
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
Tested applications
Suitable for: ICC, ELISA, WB, IHC-Pmore details -
Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
Conjugation notes
Molar enzyme: antibody protein ratio is 1:1 -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Part of the AbExcel range. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent dilution.
|
|
| ELISA |
1/5000 - 1/50000. (Primary ELISA)
|
|
| WB | (1) |
1/5000 - 1/50000. (Chemiluminescent)
|
| IHC-P |
1/100 - 1/1000.
|
| Notes |
|---|
|
ICC
Use at an assay dependent dilution. |
|
ELISA
1/5000 - 1/50000. (Primary ELISA) |
|
WB
1/5000 - 1/50000. (Chemiluminescent) |
|
IHC-P
1/100 - 1/1000. |
Images
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Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020)All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/5000 dilution
Lanes 3-4 : Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/20000 dilution
Lanes 5-6 : Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 4 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab97020 has been referenced in 23 publications.
- Whittle L et al. Characterization of a Novel Chimeric Theileria parva p67 Antigen Which Incorporates into Virus-like Particles and Is Highly Immunogenic in Mice. Vaccines (Basel) 10:N/A (2022). PubMed: 35214669
- Kariyazono R & Osanai T Identification of the genome-wide distribution of cyanobacterial group-2 sigma factor SigE, accountable for its regulon. Plant J 110:548-561 (2022). PubMed: 35092706
- Sun Y et al. Force-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in Mice. Stem Cells Int 2022:4775445 (2022). PubMed: 36110889
- Sadoch J et al. High-throughput evolutionary optimization of the induction medium towards recombinant protein production in BY-2 tobacco. Biotechnol Bioeng 118:676-689 (2021). PubMed: 33038011
- Gardner AI et al. Interferon regulatory factor 8 regulates expression of acid ceramidase and infection susceptibility in cystic fibrosis. J Biol Chem 296:100650 (2021). PubMed: 33839155