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Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020)

Reviews (1)Q&A (2)References (19)
Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020)

    Key features and details

    • Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)
    • Conjugation: Alkaline Phosphatase
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: ICC, ELISA, WB, IHC-P

    Conjugates logo Related conjugates and formulations

    Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 10nm Gold 6nm HRP HRP polymer IRDye® 680RD PE Texas Red ® TRITC Unconjugated Unconjugated

    Overview

    • Product name

      Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)
      See all IgG secondary antibodies

    • Host species

      Goat

    • Target species

      Mouse

    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

    • Tested applications

      Suitable for: ICC, ELISA, WB, IHC-Pmore details

    • Conjugation

      Alkaline Phosphatase

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).

    • Conjugation notes

      Molar enzyme: antibody protein ratio is 1:1

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • General notes

      Part of the AbExcel range.

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab97020 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC
    Use at an assay dependent dilution.
    ELISA
    1/5000 - 1/50000. (Primary ELISA)
    WB (1)
    1/5000 - 1/50000. (Chemiluminescent)
    IHC-P
    1/100 - 1/1000.
    Notes
    ICC
    Use at an assay dependent dilution.
    ELISA
    1/5000 - 1/50000. (Primary ELISA)
    WB
    1/5000 - 1/50000. (Chemiluminescent)
    IHC-P
    1/100 - 1/1000.

    Images

    • Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020)
      Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020)
      All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

      All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1-2 : Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/5000 dilution
      Lanes 3-4 : Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/20000 dilution
      Lanes 5-6 : Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 4 minutes

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (19)

    Publishing research using ab97020? Please let us know so that we can cite the reference in this datasheet.

    ab97020 has been referenced in 19 publications.

    • Whittle L  et al. Characterization of a Novel Chimeric Theileria parva p67 Antigen Which Incorporates into Virus-like Particles and Is Highly Immunogenic in Mice. Vaccines (Basel) 10:N/A (2022). PubMed: 35214669
    • Ahsan A  et al. Characterization of linear epitope specificity of antibodies potentially contributing to spontaneous clearance of hepatitis C virus. PLoS One 16:e0256816 (2021). PubMed: 34449828
    • Kong W  et al. mRNA surveillance complex PELOTA-HBS1 regulates phosphoinositide-dependent protein kinase1 and plant growth. Plant Physiol 186:2003-2020 (2021). PubMed: 33930167
    • Tartor H  et al. Quantification of Defensive Proteins in Skin Mucus of Atlantic salmon Using Minimally Invasive Sampling and High-Sensitivity ELISA. Animals (Basel) 10:N/A (2020). PubMed: 32784772
    • Holthaus D  et al. Direct Antiviral Activity of IFN-Stimulated Genes Is Responsible for Resistance to Paramyxoviruses in ISG15-Deficient Cells. J Immunol 205:261-271 (2020). PubMed: 32423918
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Tubulin

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    5 ug of cell spermatocyte lysate with RIPA buffer
    30 min skim milk blocking
    Primary 1:2000 room temp for 1 hr
    Secondary 1:2000 Room temp

    Detection with Alkaline phosphatase substrate
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 06 2016

    Question

    Hello

    I wonder if you could help me I am urgently looking to source the below two products. Could you advise your part numbers for these?

    6x-His Epitope Tag Monoclonal Antibody and Goat anti-Mouse IgG Secondary Antibody, AP conjugate

    Thank you for all your help.

    Kind Regards

    Read More
    Answer

    Thank you for contacting us.

    Please check the catalogue number ab5000; https://www.abcam.com/6X-His-tag-antibody-4D11-ab5000.html

    ab97020; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-HL-AP-ab97020.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    To technical support,



    I would like to order a complete set of antibodies and controls from Abcam for WB analysis. Could you check if these antibodies would suit for the analysis. In addition, could you recommend some positive controls.







    https://www.abcam.com/P-Glycoprotein-antibody-JSB-1-ab3366.html



    https://www.abcam.com/P-Glycoprotein-antibody-JSB-1-ab3366.html





    https://www.abcam.com/MRP2-antibody-M2-III-6-ab3373.html



    https://www.abcam.com/MRP2-antibody-M2-III-6-ab3373.html





    https://www.abcam.com/MRP3-antibody-M3II-9-ab3375.html



    https://www.abcam.com/MRP3-antibody-M3II-9-ab3375.html





    https://www.abcam.com/MRP1-antibody-MRPm5-ab24102.html



    https://www.abcam.com/MRP1-antibody-MRPm5-ab24102.html





    https://www.abcam.com/BCRPABCG2-antibody-ab63907.html



    https://www.abcam.com/BCRPABCG2-antibody-ab63907.html





    https://www.abcam.com/MRP4-antibody-ab77184.html



    https://www.abcam.com/MRP4-antibody-ab77184.html





    https://www.abcam.com/Goat-Mouse-IgG-HL-Alkaline-Phosphatase-ab97020.html



    https://www.abcam.com/Goat-Mouse-IgG-HL-Alkaline-Phosphatase-ab97020.html





    https://www.abcam.com/Goat-Rabbit-IgG-HL-Alkaline-Phosphatase-ab97048.html



    https://www.abcam.com/Goat-Rabbit-IgG-HL-Alkaline-Phosphatase-ab97048.html





    https://www.abcam.com/Rabbit-Goat-IgG-HL-Alkaline-Phosphatase-ab97097.html



    https://www.abcam.com/Rabbit-Goat-IgG-HL-Alkaline-Phosphatase-ab97097.html





    https://www.abcam.com/NBT--BCIP-Solution-Ready-to-Use-Alkaline-Phosphatase-chromogen-ab7468.html



    https://www.abcam.com/NBT--BCIP-Solution-Ready-to-Use-Alkaline-Phosphatase-chromogen-ab7468.html





    https://www.abcam.com/alpha-Tubulin-antibody-DM1A-Loading-Control-ab7291.html



    https://www.abcam.com/alpha-Tubulin-antibody-DM1A-Loading-Control-ab7291.html







    With kind regards,

    Read More
    Answer

    Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
    I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab24102.
    https://www.abcam.com/Human-liver-tissue-lysate-total-protein-ab29889.html https://www.abcam.com/Human-liver-tissue-lysate-total-protein-ab29889.html.
    MRP4 is barely detectable in liver and therefore I recommend to use ab30304 (Prostate (Human) Tissue Lysate - adult normal tissue) as positive control.
    https://www.abcam.com/Human-prostate-tissue-lysate-total-protein-ab30304.html https://www.abcam.com/Human-prostate-tissue-lysate-total-protein-ab30304.html.
    With ab63907 we used ab29745 (Placenta (Human) Tissue Lysate - adult normal tissue) successfully as control.
    https://www.abcam.com/Human-placenta-tissue-lysate-total-protein-ab29745.html https://www.abcam.com/Human-placenta-tissue-lysate-total-protein-ab29745.html.
    I hope this information is helpful and wish you good luck with your research.

    Read More

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