Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed (ab7069)

Thank you for your inquiry. I've included the link to the secondary antibody that we talked about on the phone.  This would work if your other antibody is raised in a mouse.  I've also included a link to some of our substrate kits. https://www.abcam.com/Mouse-IgG-secondary-antibody-H-L-Pre-Adsorbed-ab7069.html https://www.abcam.com/index.html?pageconfig=resource&rid=12886 https://www.abcam.com/index.html?pageconfig=resource&rid=12908 Here is the protocol from IHC world that talked about sequential double staining and a few other references: http://www.ihcworld.com/_protocols/general_IHC/immunoenzyme_double.htm http://www.ihcworld.com/_books/Dako_Handbook.pdf http://www.dako.com/08002_03aug09_ihc_guidebook_5th_edition_chapter_15.pdf This is the note I referenced about having bone sometimes come off the slide and a note about decalcification: http://www.histosearch.com/histonet/Jun99/BoneIHC.html http://www.scionpublishing.com/shop/ProductImages/Immunohistochemistry%20Chapter%204.pdf I hope this information helps.  Please contact us with any other questions.

Thank you for your enquiry. As you mentioned in your e-mail, we currently have two Goat anti-mouse AP conjugated secondary antisera: ab7069 and ab6790. ab7069 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. ab6790 has not been prepared using a preabsorbtion step. For your needs in western blotting, I feel that either sera would be suitable. Usually if the application was IHC where endogenous IgG is likely to be present at higher levels then I think the preabsorbtion of sera may prove a useful purification step. I hope this information helps. Please do not hesitate to contact me should you require further assistance.