Goat Anti-Mouse IgG H&L (APC) preadsorbed (ab130782)
Key features and details
- Goat Anti-Mouse IgG H&L (APC) preadsorbed
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, ELISA, WB
Related conjugates and formulations
Overview
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Product name
Goat Anti-Mouse IgG H&L (APC) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
ab130782 reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b and IgG3 and with the light chains of mouse IgM and IgA as demonstrated by ELISA, FLISA, and flow cytometry. Goat Anti-Mouse IgG H&L (Allophycocyanin) preadsorbed exhibits minimal cross reactivity with Human immunoglobulins.
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Tested applications
Suitable for: ICC/IF, ELISA, WBmore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Mouse IgG paraproteins
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Conjugation
APC. Ex: 645nm, Em: 660nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: PBS, Sucrose -
Concentration information loading... -
Purification notes
ab130782 was purified by affinity chromatography on pooled Mouse IgG covalently linked to agarose. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
ab130782 is cross absorbed against pooled Human sera and purified Human paraproteins. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use 0.1µg for 106 cells.
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| ELISA |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC/IF
Use 0.1µg for 106 cells. |
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ELISA
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab130782 has been referenced in 2 publications.
- Yuan X et al. Rhein enhances the cytotoxicity of effector lymphocytes in colon cancer under hypoxic conditions. Exp Ther Med 16:5350-5358 (2018). PubMed: 30542494
- Zhang Q et al. Surface expression of anti-CD3scfv stimulates locoregional immunotherapy against hepatocellular carcinoma depending on the E1A-engineered human umbilical cord mesenchymal stem cells. Int J Cancer 141:1445-1457 (2017). PubMed: 28643325