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Goat Anti-Mouse IgG H&L (Biotin) (ab97021)

Submit a review Q&A (2)References (14)

Key features and details

  • Goat Anti-Mouse IgG H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ICC/IF, ELISA, WB

Overview

  • Product name

    Goat Anti-Mouse IgG H&L (Biotin)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, ELISA, WBmore details

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

  • Conjugation notes

    Biotin/ antibody protein ratio is 4:1

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/500 - 1/5000.
ICC/IF
1/200 - 1/1000.
ELISA
1/20000 - 1/400000.
WB
1/20000 - 1/400000. Predicted molecular weight: 36 kDa.
Notes
IHC-P
1/500 - 1/5000.
ICC/IF
1/200 - 1/1000.
ELISA
1/20000 - 1/400000.
WB
1/20000 - 1/400000. Predicted molecular weight: 36 kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (14)

Publishing research using ab97021? Please let us know so that we can cite the reference in this datasheet.

ab97021 has been referenced in 14 publications.

  • Dicks AR  et al. Chondrogenic Differentiation of Human-Induced Pluripotent Stem Cells. Methods Mol Biol 2598:87-114 (2023). PubMed: 36355287
  • Pena MJ  et al. Unveiling the Metabolic Effects of Glycomacropeptide. Int J Mol Sci 22:N/A (2021). PubMed: 34575895
  • Liang Y  et al. Re-Differentiation of Human Meniscus Fibrochondrocytes Differs in Three-Dimensional Cell Aggregates and Decellularized Human Meniscus Matrix Scaffolds. Ann Biomed Eng 48:968-979 (2020). PubMed: 31147805
  • Huynh NPT  et al. High-depth transcriptomic profiling reveals the temporal gene signature of human mesenchymal stem cells during chondrogenesis. FASEB J 33:358-372 (2019). PubMed: 29985644
  • Adkar SS  et al. Step-Wise Chondrogenesis of Human Induced Pluripotent Stem Cells and Purification Via a Reporter Allele Generated by CRISPR-Cas9 Genome Editing. Stem Cells 37:65-76 (2019). PubMed: 30378731
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Dear Technical Team, Hope this e-mail finds you well! Kindly arrange to send us the certificate of compliance (CoC) for the below Abcam products as desired by one of our customers : Cat No. Description ab97021 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) ab97049 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) ab97023 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) ab60314 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) ab97080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed ab65623 ECL Western Blotting Substrate Kit - 50 Tests ab52476 Rabbit monoclonal [EP1829Y] to CaMKII ab11800 Goat polyclonal to DCXR ab7260 Rabbit polyclonal to GFAP - Astrocyte Marker ab104224 Mouse monoclonal [1B7] to FOX3 ab76260 Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9) ab64230 Mounting Medium ab82477 Rabbit polyclonal to Adenosine A1 Receptor ab3462 Rabbit polyclonal to alpha 1 Adrenergic Receptor

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Answer

I am beginning to gather the CoC's you have requested. When you have a chance, please provide the lot number from each catalog number so I can include this information on the documentation. Thanks for the additional information.

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Question

1) wie rein ist ab64255 (BSA?)? Ich moechte den AK radiomarkieren, biotin auch wichtig. 2) Konzentration/Volumen? "125ml at mg/ml" macht keinen Sinn.

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Answer

Vielen Dank für Ihren Anruf. ab64255 wurde über Affinitätschromatographie aus dem Antiserum aufgereinigt; der exakte Reinheitsgrade wurde leider nicht bestimmt. Der Puffer, in dem der Antikörper vorliegt, enthält BSA. Die Konzentration beträgt 7 µg/ml, und weil es sich um ein ready-to-use-Produkt handelt, bekommen Sie tatsächlich 125 ml ! Für Ihre Versuchsvorhaben könnte sich dieser Antikörper daher vielleicht nicht unbedingt eignen - weil sich 125 ml doch eher schlecht verarbeiten lassen. Gerne möchte ich Ihnen zwei Alternativen nennen, die in weniger Volumen erhältlich sind (ab97021 und ab97033). Beide Antikörper sind biotinyliert, ab97033 ist zusätzlich noch prae-absorbiert, d.h. er reagiert nicht mit den auf dem Datenblatt angegebenen Spezies (wie Huhn, Rind, Ziege etc): Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-Biotin-ab97021.html). Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-Biotin-preadsorbed-ab97033.html). Ich hoffe, diese Informationen helfen Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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