Goat Anti-Mouse IgG H&L (Biotin) (ab97021)
Key features and details
- Goat Anti-Mouse IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, ELISA, WB
Overview
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Product name
Goat Anti-Mouse IgG H&L (Biotin)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
Tested applications
Suitable for: IHC-P, ICC/IF, ELISA, WBmore details -
Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells. -
Conjugation notes
Biotin/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500 - 1/5000.
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ICC/IF |
1/200 - 1/1000.
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ELISA |
1/20000 - 1/400000.
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WB |
1/20000 - 1/400000. Predicted molecular weight: 36 kDa.
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Notes |
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IHC-P
1/500 - 1/5000. |
ICC/IF
1/200 - 1/1000. |
ELISA
1/20000 - 1/400000. |
WB
1/20000 - 1/400000. Predicted molecular weight: 36 kDa. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab97021 has been referenced in 14 publications.
- Dicks AR et al. Chondrogenic Differentiation of Human-Induced Pluripotent Stem Cells. Methods Mol Biol 2598:87-114 (2023). PubMed: 36355287
- Pena MJ et al. Unveiling the Metabolic Effects of Glycomacropeptide. Int J Mol Sci 22:N/A (2021). PubMed: 34575895
- Liang Y et al. Re-Differentiation of Human Meniscus Fibrochondrocytes Differs in Three-Dimensional Cell Aggregates and Decellularized Human Meniscus Matrix Scaffolds. Ann Biomed Eng 48:968-979 (2020). PubMed: 31147805
- Huynh NPT et al. High-depth transcriptomic profiling reveals the temporal gene signature of human mesenchymal stem cells during chondrogenesis. FASEB J 33:358-372 (2019). PubMed: 29985644
- Adkar SS et al. Step-Wise Chondrogenesis of Human Induced Pluripotent Stem Cells and Purification Via a Reporter Allele Generated by CRISPR-Cas9 Genome Editing. Stem Cells 37:65-76 (2019). PubMed: 30378731