Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944)
Key features and details
- Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed
- Conjugation: Cy2 ®. Ex: 489nm, Em: 505nm
- Host species: Goat
- Suitable for: ICC/IF, IHC-P, ELISA, WB, Flow Cyt, IHC-Fr
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Overview
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Product name
Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-P, ELISA, WB, Flow Cyt, IHC-Frmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
Immunogen
Full length native protein (purified) corresponding to IgG.
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Conjugation
Cy2 ®. Ex: 489nm, Em: 505nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. -
Conjugation notes
Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 5.1 moles Cy2 per mole of Goat IgG -
Clonality
Polyclonal -
General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ELISA |
1/10000 - 1/50000.
|
|
| WB |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent concentration. |
|
ELISA
1/10000 - 1/50000. |
|
WB
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
Images
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Western blot - Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944)Secondary
Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944) at 1/1000 dilution (60 minutes at Room temperature)
Observed band size: 25, 55 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab6944 has been referenced in 8 publications.
- Collins C et al. Tubulin acetylation promotes penetrative capacity of cells undergoing radial intercalation. Cell Rep 36:109556 (2021). PubMed: 34407402
- Piao CS et al. Depression following traumatic brain injury in mice is associated with down-regulation of hippocampal astrocyte glutamate transporters by thrombin. J Cereb Blood Flow Metab 39:58-73 (2019). PubMed: 29135354
- Yamazaki T et al. Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation. Mol Cell 70:1038-1053.e7 (2018). PubMed: 29932899
- Hartman CL et al. 2-Chlorofatty acids induce Weibel-Palade body mobilization. J Lipid Res 59:113-122 (2018). PubMed: 29167411
- Gümürdü A et al. MicroRNA exocytosis by large dense-core vesicle fusion. Sci Rep 7:45661 (2017). PubMed: 28358390
- Wen SY et al. In vitro neuroprotective effects of ciliary neurotrophic factor on dorsal root ganglion neurons with glutamate-induced neurotoxicity. Neural Regen Res 12:1716-1723 (2017). IF . PubMed: 29171438
- West JA et al. Structural, super-resolution microscopy analysis of paraspeckle nuclear body organization. J Cell Biol 214:817-30 (2016). PubMed: 27646274
- Marbach J et al. Expression and characterisation of fully posttranslationally modified cellular prion protein in Pichia pastoris. Biol Chem 394:1475-83 (2013). PubMed: 23893688