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Goat Anti-Mouse IgG H&L (Cy5.5 ®) preadsorbed (ab6947)

Reviews (1)Q&A (5)References (9)

Key features and details

  • Goat Anti-Mouse IgG H&L (Cy5.5 ®) preadsorbed
  • Conjugation: Cy5.5 ®. Ex: 678nm, Em: 703nm
  • Host species: Goat
  • Suitable for: ICC/IF, ELISA, Flow Cyt, IHC-P, IHC-Fr, Immunomicroscopy

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 10nm Gold 6nm HRP HRP polymer IRDye® 680RD PE Texas Red ® TRITC Unconjugated Unconjugated

Overview

  • Product name

    Goat Anti-Mouse IgG H&L (Cy5.5 ®) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, ELISA, Flow Cyt, IHC-P, IHC-Fr, Immunomicroscopymore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • Immunogen

    Full length native Mouse IgG (purified).

  • Conjugation

    Cy5.5 ®. Ex: 678nm, Em: 703nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.

  • Conjugation notes

    Cy5.5 (Cyanine 5.5-OSu) (Molecular Weight 1312 daltons) Absorption Wavelength: 678 nm Emission Wavelength: 703 nm Fluorochrome/Protein Ratio: 3.7 moles Cy5.5 per mole of Goat IgG

  • Clonality

    Polyclonal

  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000 - 1/5000.
ELISA
1/10000 - 1/50000.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
Notes
ICC/IF
1/1000 - 1/5000.
ELISA
1/10000 - 1/50000.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (9)

Publishing research using ab6947? Please let us know so that we can cite the reference in this datasheet.

ab6947 has been referenced in 9 publications.

  • Afanasyeva EA  et al. Kalirin-RAC controls nucleokinetic migration in ADRN-type neuroblastoma. Life Sci Alliance 4:N/A (2021). PubMed: 33658318
  • Bauwens S  et al. The Telomeric Protein TRF2 Regulates Replication Origin Activity within Pericentromeric Heterochromatin. Life (Basel) 11:N/A (2021). PubMed: 33804994
  • Ben Yamin B  et al. DNA polymerase zeta contributes to heterochromatin replication to prevent genome instability. EMBO J 40:e104543 (2021). PubMed: 34533226
  • Mendez-Bermudez A  et al. Genome-wide Control of Heterochromatin Replication by the Telomere Capping Protein TRF2. Mol Cell 70:449-461.e5 (2018). PubMed: 29727617
  • Holloway PM  et al. Sulforaphane induces neurovascular protection against a systemic inflammatory challenge via both Nrf2-dependent and independent pathways. Vascul Pharmacol 85:29-38 (2016). PubMed: 27401964
  • Wilhelm T  et al. Spontaneous slow replication fork progression elicits mitosis alterations in homologous recombination-deficient mammalian cells. Proc Natl Acad Sci U S A 111:763-8 (2014). PubMed: 24347643
  • Miotto B  et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). ICC/IF . PubMed: 24726359
  • Técher H  et al. Replication dynamics: biases and robustness of DNA fiber analysis. J Mol Biol 425:4845-55 (2013). PubMed: 23557832
  • Letessier A  et al. Cell-type-specific replication initiation programs set fragility of the FRA3B fragile site. Nature 470:120-3 (2011). IF . PubMed: 21258320

Customer reviews and Q&As

Show All Reviews Q&A
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1-6 of 6 Abreviews or Q&A

Poor detection of mouse IgG in rat brain with significant background

 Poor
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Image is a subsection of rat brain with ventricle and white matter tracts visible. In sections with primary antibody, a 1:125 dilution of secondary antibody resulted in very weak signal. In this image, only secondary antibody was added at the same dilution (i.e. secondary-only control) and developed a strong background signal of white matter tracts. Cell nuclei were counterstained with DAPI (blue).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 24 2021

Question

Yes, it is correct. ************does the purchasing in this group usually. I am a visiting professor here.

Feel free to contact him to double-check, he is the one who gave me the customer ID.

Read More
Answer

Thank you for your reply.

I have sent the quote for the products requested to the email address we have on file for **********.

When you are ready to convert the quote to an order, please contact our customer service team on 1-888-772-2226 or email to mailto:us.orders@abcam.com.

If there is anything else I can help you with, please let me know.

Read More

Question

Concerning the quote, the customer number is**********

Read More
Answer

Thank you for your reply.

Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just wanted to make sure that the quote went to the correct person.

Thank you for your patience, and I look forward to your reply.

Read More

Question

Can you please read my email again?

Read More
Answer

Yes, you can use ab6947 with ab54427. The antibody that recognizes IgG will recognize all IgG subtypes, whereas the IgG1, will only recognize IgG. Therefore, if you are not intending to use any other mouse primary antibodies at the same time then the IgG antibody will work well, if however you would like to use other mouse antibodies, then I would recommend the IgG1.

Please let me know if there is anything else I can help you with, and if you can provide me the address, I can generate a quote.

Read More

Question

I selected the following antibodies from the ABcam catalog:

Primary:

Anti-CD63 antibody [AD1] (FITC)

ab108949

$337.00



Anti-CD62P antibody [Psel.KO.2.7]

ab54427

$337.00



Anti-CD42d antibody [1C2]

ab95780

$337.00



Secondary:


Goat anti-Mouse IgG H&L (Cy5.5 ®)

ab6947

$185.00



Goat anti-Mouse IgG1 heavy chain (FITC)

ab97239

$165.00



Goat polyclonal to Armenian Hamster (TR)

ab5743

$165.00



Isotype controls:


MOUSE IgG1 antibody [MOPC-21] (FITC)

ab106163

$325.00



Mouse IgG1 [ICIGG1] - Isotype Control

ab91353

$315.00



Armenian Hamster IgG monoclonal [HTK888] - Isotype control

ab18479

$325.00



Two questions:

For the secondary antibody, will the first one (ab6947) work against ab54427, or do we need to buy the second one (ab97239)? One is against IgG, the other one is against IgG1. ab54427 is IgG1.

If you could double-check the selection for compatibility (e.g., correct isotype controls) and then ask the sales department to offer us a quote, that would be great.

Read More
Answer

Thank you for your reply.

The antibodies you selected look good to me. To generate a quote we do need to have a shipping address and in your last email, you mentioned that you were at the University of Toronto at the moment. Could you please send me your current email address and I will generate the quote.

I look forward to your reply.

Read More

Question

Would you please tell me who is the manufacturer of this product?

Read More
Answer

Thank you for your enquiry. We produce some of our antibodies in-house and others are sourced from suppliers whose products we believe will be of interest to our customers. This product falls into the latter category. The identity of our suppliers remains commercially sensitive information. If you have any additional questions, please contact us again.

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