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    products/secondary-antibodies/goat-mouse-igg-hl-dylight-550-preadsorbed-ab96880.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab96880)

  • Datasheet
Submit a review Q&A (1)References (10)

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Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab96880)

    Key features and details

    • Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed
    • Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: IHC-P, ICC/IF, Flow Cyt

    Conjugates logo Related conjugates and formulations

    Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 10nm Gold 6nm HRP HRP polymer IRDye® 680RD PE Texas Red ® TRITC Unconjugated Unconjugated

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    Overview

    • Product name

      Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed
      See all IgG secondary antibodies
    • Host species

      Goat
    • Target species

      Mouse
    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

       

    • Tested applications

      Suitable for: IHC-P, ICC/IF, Flow Cytmore details
    • Minimal
      cross-reactivity


      Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat
      To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
      more details
    • Conjugation

      DyLight® 550. Ex: 562nm, Em: 576nm

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • General notes

      DyLight® 550 replaces DyLight® 549.
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Mouse
      • IgG
      • Fluorophore
      • DyLight® 550

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab96880 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/50 - 1/500.

    DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.

    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.
    Notes
    IHC-P
    1/50 - 1/500.

    DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.

    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.

    Images

    • Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab96880)
      Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab96880)
      Emission spectra of DyLight® fluorochromes available in our catalog.
      Line colors represent the approximate visible colors of the wavelength maxima.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (10)

    Publishing research using ab96880? Please let us know so that we can cite the reference in this datasheet.

    ab96880 has been referenced in 10 publications.

    • Nguyen KDQ  et al. Homo-oligomerization of the human adenosine A2A receptor is driven by the intrinsically disordered C-terminus. Elife 10:N/A (2021). PubMed: 34269678
    • Mannino G  et al. ARPE-19 conditioned medium promotes neural differentiation of adipose-derived mesenchymal stem cells. World J Stem Cells 13:1783-1796 (2021). PubMed: 34909123
    • Klepinin A  et al. The complexity of mitochondrial outer membrane permeability and VDAC regulation by associated proteins. J Bioenerg Biomembr N/A:N/A (2018). PubMed: 29998379
    • Wagener KC  et al. Redox Indicator Mice Stably Expressing Genetically Encoded Neuronal roGFP: Versatile Tools to Decipher Subcellular Redox Dynamics in Neuropathophysiology. Antioxid Redox Signal 25:41-58 (2016). ICC/IF . PubMed: 27059697
    • Dorris ER  et al. Pluripotency markers are differentially induced by MEK inhibition in thyroid and melanoma BRAFV600E cell lines. Cancer Biol Ther 17:526-42 (2016). WB . PubMed: 26828826
    • Schonenbach NS  et al. Adenosine A2a receptors form distinct oligomers in protein detergent complexes. FEBS Lett 590:3295-306 (2016). PubMed: 27543907
    • Varikmaa M  et al. Role of mitochondria-cytoskeleton interactions in respiration regulation and mitochondrial organization in striated muscles. Biochim Biophys Acta 1837:232-45 (2014). PubMed: 24189374
    • Aldrin-Kirk P  et al. Novel AAV-based rat model of forebrain synucleinopathy shows extensive pathologies and progressive loss of cholinergic interneurons. PLoS One 9:e100869 (2014). PubMed: 24999658
    • Kaldma A  et al. An in situ study of bioenergetic properties of human colorectal cancer: The regulation of mitochondrial respiration and distribution of flux control among the components of ATP synthasome. Int J Biochem Cell Biol 55C:171-186 (2014). IHC-P . PubMed: 25218857
    • Varikmaa M  et al. Matters of the heart in bioenergetics: mitochondrial fusion into continuous reticulum is not needed for maximal respiratory activity. J Bioenerg Biomembr 45:319-31 (2013). PubMed: 23271420

    Customer reviews and Q&As

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    Question

    Could you confirm if any of the dylight dyes are brighter than other dyes available for around 568 nm (eg alexa 568 nm)

    Read More

    Abcam community

    Verified customer

    Asked on Dec 02 2011

    Answer

    Thank you for your recent telephone enquiry. I am forwarding a link to our Dyelight information page from the Abcam website: https://www.abcam.com/index.html? pageconfig=resource&rid=12934#2 I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Dec 02 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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