Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881)
Key features and details
- Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed
- Conjugation: DyLight® 594. Ex: 593nm, Em: 618nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
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Tested applications
Suitable for: ICC/IF, IHC-P, Flow Cytmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat more details -
Immunogen
Other Immunogen Type corresponding to Mouse IgG.
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Conjugation
DyLight® 594. Ex: 593nm, Em: 618nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/500.
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IHC-P |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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Notes |
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ICC/IF
1/50 - 1/500. |
IHC-P
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
Images
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881)
ab96881 was used at dilution 1/100 for IHC analysis of FFPE human kidney. Primary Antibody: mouse anti-p53 (clone DO-1) used at a dilution of 1/100. Secondary Antibody: Red-fluorescent goat anti-mouse IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated ab96881 (2 different lots ) used at a dilution of 1/100 (5µg/mL). Counterstain: DAPI (blue)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (22)
ab96881 has been referenced in 22 publications.
- Wu N et al. Essential role of MALAT1 in reducing traumatic brain injury. Neural Regen Res 17:1776-1784 (2022). PubMed: 35017438
- Shi Y et al. ROS Promote Hypoxia-Induced Keratinocyte Epithelial-Mesenchymal Transition by Inducing SOX2 Expression and Subsequent Activation of Wnt/ß-Catenin. Oxid Med Cell Longev 2022:1084006 (2022). PubMed: 35035654
- Selinger M et al. Integrative RNA profiling of TBEV-infected neurons and astrocytes reveals potential pathogenic effectors. Comput Struct Biotechnol J 20:2759-2777 (2022). PubMed: 35685361
- Selinger M et al. Tick-borne encephalitis virus capsid protein induces translational shutoff as revealed by its structural-biological analysis. J Biol Chem 298:102585 (2022). PubMed: 36223838
- Wang W et al. Electrofusion Stimulation Is an Independent Factor of Chromosome Abnormality in Mice Oocytes Reconstructed via Spindle Transfer. Front Endocrinol (Lausanne) 12:705837 (2021). PubMed: 34413830