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Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881)

Submit a review Q&A (1)References (22)
Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881)

Key features and details

  • Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed
  • Conjugation: DyLight® 594. Ex: 593nm, Em: 618nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, Flow Cyt

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 650 FITC Glucose Oxidase Gold 10nm Gold 6nm HRP HRP polymer IRDye® 680RD PE Texas Red ® TRITC Unconjugated Unconjugated

Overview

  • Product name

    Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

     

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cytmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat more details

  • Immunogen

    Other Immunogen Type corresponding to Mouse IgG.

  • Conjugation

    DyLight® 594. Ex: 593nm, Em: 618nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab96881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50 - 1/500.
IHC-P
1/50 - 1/500.
Flow Cyt
1/50 - 1/200.
Notes
ICC/IF
1/50 - 1/500.
IHC-P
1/50 - 1/500.
Flow Cyt
1/50 - 1/200.

Images

  • Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881)
    Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881)

    ab96881 was used at dilution 1/100 for IHC analysis of FFPE human kidney. Primary Antibody: mouse anti-p53 (clone DO-1) used at a dilution of 1/100. Secondary Antibody: Red-fluorescent goat anti-mouse IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated ab96881 (2 different lots ) used at a dilution of 1/100 (5µg/mL). Counterstain: DAPI (blue)

     

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (22)

Publishing research using ab96881? Please let us know so that we can cite the reference in this datasheet.

ab96881 has been referenced in 22 publications.

  • Wu N  et al. Essential role of MALAT1 in reducing traumatic brain injury. Neural Regen Res 17:1776-1784 (2022). PubMed: 35017438
  • Shi Y  et al. ROS Promote Hypoxia-Induced Keratinocyte Epithelial-Mesenchymal Transition by Inducing SOX2 Expression and Subsequent Activation of Wnt/ß-Catenin. Oxid Med Cell Longev 2022:1084006 (2022). PubMed: 35035654
  • Selinger M  et al. Integrative RNA profiling of TBEV-infected neurons and astrocytes reveals potential pathogenic effectors. Comput Struct Biotechnol J 20:2759-2777 (2022). PubMed: 35685361
  • Selinger M  et al. Tick-borne encephalitis virus capsid protein induces translational shutoff as revealed by its structural-biological analysis. J Biol Chem 298:102585 (2022). PubMed: 36223838
  • Wang W  et al. Electrofusion Stimulation Is an Independent Factor of Chromosome Abnormality in Mice Oocytes Reconstructed via Spindle Transfer. Front Endocrinol (Lausanne) 12:705837 (2021). PubMed: 34413830
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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Question

Dears,   I know those items below are from a competitor! Do we have any of those below?   Would you please send me the quote for: 1.  Alexa488 phalloidin, vial 2.  Alexa594 phalloidin, vial 3.  Coat anti mouse secondary antibody conjugated to alexa 594 4.  Goat anti mouse secondary antibody conjugated to Alexa 488 5.  Alexa fluor 488 protein labeling kit 6.  Alexa fluor 594 protein labeling kit 7.  Goat anti rabbit secondary antibody conjugated to alexa 488, vial 8.  Goat anti rabbit secondary antibody conjugated to Alexi 594 , vial   Thanks !    

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Answer

Thank you for your email and interest in Abcam products. We currently do not have AlexaFluor conjugated antibodies or proteins available in our catalog. Having said this though, we have a number of products available that are conjugated to DyLight® fluorochromes. This family of dyes is comparable to AlexaFluor dyes regarding high fluorescence intensity, photostability and water solubility. Both the absorption and emission properties of the DyLight® fluorochomes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation (https://www.abcam.com/DyLight). Please be reassured that all our products are covered by our Abpromise: We guarantee our products work in the tested species and applications as stated on the datasheet, and we will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase. Thus, please see a selection of our options: 1+2) Unfortunately, we currently do not have any phalloidin protein or antibody against phalloidin available. 3) ab96873, ab96881: Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-594-ab96873.html). Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-594-preadsorbed-ab96881.html). 4) ab96871, ab96871, ab97022: Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-ab96871.html). Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-ab96871.html). Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-FITC-ab97022.html). 5) ab102883, ab102884, ab102885 (please see https://www.abcam.com/EasyLink or attachment for more details): Click here (or use the following: https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-1-x-1mg-FITC-ab102883.html). Click here (or use the following: https://www.abcam.com/FITC-Conjugation-Kit-Lightning-Linkreg-ab102884.html). Click here (or use the following: https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-10g-FITC-ab102885.html). 6) ab102923, ab1029234, ab102925 (please see https://www.abcam.com/EasyLink or attachment for more details): Click here (or use the following: https://www.abcam.com/EasyLink-Texas-Red-Conjugation-Kit-1-x-1mg-TR-ab102923.html). Click here (or use the following: https://www.abcam.com/Texas-Redreg-Conjugation-Kit-ab102924.html). Click here (or use the following: https://www.abcam.com/EasyLink-Texas-Red-Conjugation-Kit-3-x-10g-TR-ab102925.html). 7) ab97050, ab96883, ab96899: Click here (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-FITC-ab97050.html). Click here (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-488-ab96883.html). Click here (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96899.html). 8) ab96885, ab96897: Click here (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-594-ab96885.html). Click here (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-594-preadsorbed-ab96897.html). We also have the secondary antibodies in different formats (pre-absorbed, Fab fragments etc) which might be more suitable for certain applications. I will be happy to assist you in finding the most appropriate product on request. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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