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Goat Anti-Mouse IgG H&L (DyLight® 650) preadsorbed (ab96882)

Reviews (1)Q&A (1)References (4)
Goat Anti-Mouse IgG H&L (DyLight® 650) preadsorbed (ab96882)

    Key features and details

    • Goat Anti-Mouse IgG H&L (DyLight® 650) preadsorbed
    • Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: Flow Cyt, ICC/IF, IHC-P

    Conjugates logo Related conjugates and formulations

    Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 FITC Glucose Oxidase Gold 10nm Gold 6nm HRP HRP polymer IRDye® 680RD PE Texas Red ® TRITC Unconjugated Unconjugated

    Overview

    • Product name

      Goat Anti-Mouse IgG H&L (DyLight® 650) preadsorbed
      See all IgG secondary antibodies

    • Host species

      Goat

    • Target species

      Mouse

    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

       

    • Tested applications

      Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details

    • Minimal
      cross-reactivity


      Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat more details

    • Immunogen

      Other Immunogen Type corresponding to Mouse IgG.

    • Conjugation

      DyLight® 650. Ex: 654nm, Em: 673nm

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • General notes

      DyLight® 650 replaces DyLight® 649.

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab96882 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    1/50 - 1/200.
    ICC/IF (1)
    1/50 - 1/500.
    IHC-P
    1/50 - 1/500.

    DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.
    Notes
    Flow Cyt
    1/50 - 1/200.
    ICC/IF
    1/50 - 1/500.
    IHC-P
    1/50 - 1/500.

    DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.

    Images

    • Goat Anti-Mouse IgG H&L (DyLight® 650) preadsorbed (ab96882)
      Goat Anti-Mouse IgG H&L (DyLight® 650) preadsorbed (ab96882)
      Emission spectra of DyLight® fluorochromes available in our catalog.
      Line colors represent the approximate visible colors of the wavelength maxima.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (4)

    Publishing research using ab96882? Please let us know so that we can cite the reference in this datasheet.

    ab96882 has been referenced in 4 publications.

    • Li HY  et al. C-Reactive Protein Protects Against Acetaminophen-Induced Liver Injury by Preventing Complement Overactivation. Cell Mol Gastroenterol Hepatol 13:289-307 (2022). PubMed: 34536564
    • Martini C  et al. CD36 promotes vasculogenic mimicry in melanoma by mediating adhesion to the extracellular matrix. BMC Cancer 21:765 (2021). PubMed: 34215227
    • Flanagan SP  et al. Nonspecific nuclear uptake of anti-MUC1 aptamers by dead cells: the role of cell viability monitoring in aptamer targeting of membrane-bound protein cancer biomarkers. Anal Methods 13:1191-1203 (2021). PubMed: 33605950
    • Smith SE  et al. Chicken interferon-inducible transmembrane protein 3 restricts influenza viruses and lyssaviruses in vitro. J Virol 87:12957-66 (2013). Flow Cyt . PubMed: 24067955

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    work well on HEK293 cell

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    ab96882 is used to detect mouse transfferin receptor (red channel), After primary anibody incubation, ab96882 is incubated at room temperature in 2%BSA/PBS for 1h. Confocal imaging showing nice punctate staining on live cells.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Ya Zhou

    Verified customer

    Submitted Jan 29 2014

    Question

    LOT NUMBER XXXX ORDER NUMBER XXXXX DESCRIPTION OF THE PROBLEM No staining SAMPLE H1299 PRIMARY ANTIBODY Anti-Myc/ 1:100 / in 1 X PBS / 1hr Wash Buffer : 1 x PBS Number of washes : 5min x 3 DETECTION METHOD Immunofluorescence microscopy POSITIVE AND NEGATIVE CONTROLS USED - ANTIBODY STORAGE CONDITIONS 4C FIXATION OF SAMPLE PFA/formalin fixed paraffin embedded sections ANTIGEN RETRIEVAL - PERMEABILIZATION STEP 0.25% Triton x 100 in PBS / 10min / RT BLOCKING CONDITIONS 2% BSA in PBS / 30hr / RT Endogenous peroxidases blocked? NO Endogenous biotins blocked? NO SECONDARY ANTIBODY ab 96882 Concentration or dilution : 1:100 Diluent buffer : 2% BSA Incubation time : 1hr - 3hr Wash Buffer : 1 x PBS Number of washes : 5min x 3 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Incubation time was increased ADDITIONAL NOTES -

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    Answer

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I would like to investigate this particular case further for you, and also obtain more information for our quality records. In order to proceed with this, there are a few things I would like to clarify to better understand and solve the problems experienced with this antibody.

    -In which species is the primary antibody raised?

    -Have you tested different dilutions combinations?

    -Was a positive control used to assess the antibodies performance?

    -If fixation was performed with formalin in paraffin embedded sections, antigen retrieval may be needed. Was the antigen retrieved at all after fixation?

    -It would be very useful to test the secondary antibody with a different primary, as well as performing detecting the primary with a different secondary, to help to find out the source of the problem.

    -Incubating the primary at 4C overnight could also give better staining results.

    I would appreciate if you could provide further details, and encourage you to perform the mentioned suggestions in order to improve the results.

    In any case, as the product is still under our guarantee, please don’t hesitate to contact us if the results do not improve.

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