Goat Anti-Mouse IgG H&L (FITC) (ab6785)
Key features and details
- Goat Anti-Mouse IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WB
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Overview
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Product name
Goat Anti-Mouse IgG H&L (FITC)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WBmore details -
Immunogen
mouse whole molecule
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC (PFA fixed) |
1/1000.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
1/500 - 1/2500. PubMed: 17989178
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365.
|
|
| ICC/IF | (1) |
1/1000 - 1/5000. (PMID16006199)
|
| ELISA |
1/10000 - 1/50000.
|
|
| WB |
1/10000. PubMed: 17217624
|
| Notes |
|---|
|
IHC (PFA fixed)
1/1000. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
Flow Cyt
1/500 - 1/2500. PubMed: 17989178 |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365. |
|
ICC/IF
1/1000 - 1/5000. (PMID16006199) |
|
ELISA
1/10000 - 1/50000. |
|
WB
1/10000. PubMed: 17217624 |
Images
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785)This image is courtesy of an Abreview submitted by J Chai.
ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.
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Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) (ab6785) -
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (397)
ab6785 has been referenced in 397 publications.
- Pantaleão LC et al. Maternal diet-induced obesity during pregnancy alters lipid supply to mouse E18.5 fetuses and changes the cardiac tissue lipidome in a sex-dependent manner. Elife 11:N/A (2022). PubMed: 35025731
- Qiu F et al. Circ_0000658 knockdown inhibits epithelial-mesenchymal transition in bladder cancer via miR-498-induced HMGA2 downregulation. J Exp Clin Cancer Res 41:22 (2022). PubMed: 35031054
- Nkune NW et al. Synthesis of a novel nanobioconjugate for targeted photodynamic therapy of colon cancer enhanced with cannabidiol. Oncotarget 13:156-172 (2022). PubMed: 35070080
- Liu S et al. CD4+ T Cells Promote IgG Production in MHC-Independent and ICAM-1-Dependent Manners in Pristane-Induced Lupus Mice. Mediators Inflamm 2022:9968847 (2022). PubMed: 35095344
- Zhao F et al. Synergistic effects of ISL1 and KDM6B on non-alcoholic fatty liver disease through the regulation of SNAI1. Mol Med 28:12 (2022). PubMed: 35100965