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Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)

Reviews (2) Submit a question References (8)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)

    Key features and details

    • Goat Anti-Mouse IgG H&L (HRP polymer)
    • Conjugation: HRP polymer
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: Immunoelectrophoresis, IHC-P

    Conjugates logo Related conjugates and formulations

    Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 10nm Gold 6nm HRP IRDye® 680RD PE Texas Red ® TRITC Unconjugated Unconjugated

    Overview

    • Product name

      Goat Anti-Mouse IgG H&L (HRP polymer)
      See all IgG secondary antibodies

    • Host species

      Goat

    • Target species

      Mouse

    • Specificity

      Based on IEP, ab214879 reacts with heavy (gamma) chains on mouse IgG, light chains on all mouse immunoglobulins. No cross-reactivity is observed to non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig or rabbit.

    • Tested applications

      Suitable for: Immunoelectrophoresis, IHC-Pmore details

    • Immunogen

      Full length protein corresponding to Mouse IgG. (Purified Mouse IgG, whole molecule).

    • Conjugation

      HRP polymer

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      Preservative: 0.1% Proclin 300
      Constituents: 1% BSA, 98% PBS

      ab214879 is liquid with light red color.
    • Concentration information loading...

    • Purity

      Affinity purified

    • Purification notes

      ab214879 was affinity purified using solid phase Mouse IgG. Purity >95% by SDS-PAGE.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab214879 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Immunoelectrophoresis
    Use at an assay dependent concentration.
    IHC-P (1)
    Use at an assay dependent concentration.

    This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour.
    Notes
    Immunoelectrophoresis
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.

    This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour.

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)

      Immunohistochemical analysis of Triple positive breast cancer tissue stained with Ki-67 and detected with ab214879.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (8)

    Publishing research using ab214879? Please let us know so that we can cite the reference in this datasheet.

    ab214879 has been referenced in 8 publications.

    • Kobayashi H  et al. The Balance of Stromal BMP Signaling Mediated by GREM1 and ISLR Drives Colorectal Carcinogenesis. Gastroenterology 160:1224-1239.e30 (2021). PubMed: 33197448
    • Roper SJ  et al. 3D spheroid models of paediatric SHH medulloblastoma mimic tumour biology, drug response and metastatic dissemination. Sci Rep 11:4259 (2021). PubMed: 33608621
    • Zhu YD  et al. Neobavaisoflavone Induces Bilirubin Metabolizing Enzyme UGT1A1 via PPARa and PPAR?. Front Pharmacol 11:628314 (2020). PubMed: 33628187
    • Lucafò M  et al. miR-331-3p is involved in glucocorticoid resistance reversion by rapamycin through suppression of the MAPK signaling pathway. Cancer Chemother Pharmacol 86:361-374 (2020). PubMed: 32776229
    • Zarbakhsh S  et al. Irisin protects the substantia nigra dopaminergic neurons in the rat model of Parkinson's disease. Iran J Basic Med Sci 22:722-728 (2019). PubMed: 32373292
    • Zhang C  et al. FOXA1 is upregulated in glioma and promotes proliferation as well as cell cycle through regulation of cyclin D1 expression. Cancer Manag Res 10:3283-3293 (2018). PubMed: 30233244
    • Li F  et al. Blocking the CD47-SIRPa axis by delivery of anti-CD47 antibody induces antitumor effects in glioma and glioma stem cells. Oncoimmunology 7:e1391973 (2018). PubMed: 29308321
    • Fang L  et al. Resistance to epithelial growth factor receptor tyrosine kinase inhibitors in a patient with transformation from lung adenocarcinoma to small cell lung cancer: A case report. Oncol Lett 14:593-598 (2017). PubMed: 28693210

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Goat anti-mouse IgG (HRP polymer) works well for FFPE human sample

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    In this study to test how well the goat anti-mouse IgG (HRP polymer) works on FFPE human tissue sections, the FFPE human skin tumor section was deparaffinized and boiled in antigen retrieval buffer, 10mM Sodium Citrate pH6. Overnight incubation in a mouse anti-Smooth muscle actin primary antibody from Sigma Aldrich. The goat anti-mouse IgG (HRP polymer) secondary antibody is used at 1:1 dilution in 2% BSA for 90 minutes at room temperature. The slides were then developed in DAB solution and counter-stained with hematoxylin.
    The staining works well.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 30 2018

    Goat anti-mouse IgG (HRP polymer) works well in pig tissue

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry paraffin embedded sections
    In this study to test how well the goat anti-mouse IgG (HRP polymer) works on FFPE pig tissue sections, the FFPE human pig lung section was deparaffinized and boiled in antigen retrieval buffer, 10mM Sodium Citrate pH6. Overnight incubation in a mouse anti-Smooth muscle actin primary antibody from Sigma Aldrich. The goat anti-mouse IgG (HRP polymer) secondary antibody is used at 1:1 dilution in 2% BSA for 90 minutes at room temperature. The slides were then developed in DAB solution and counter-stained with hematoxylin.

    The staining works well. The picture enclosed shows clear SMA staining in pig lung and a negative control insert is included for reference.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 30 2018

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