Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Key features and details
- Goat Anti-Mouse IgG H&L (HRP polymer)
- Conjugation: HRP polymer
- Host species: Goat
- Isotype: IgG
- Suitable for: Immunoelectrophoresis, IHC-P
Related conjugates and formulations
Overview
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Product name
Goat Anti-Mouse IgG H&L (HRP polymer)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
Based on IEP, ab214879 reacts with heavy (gamma) chains on mouse IgG, light chains on all mouse immunoglobulins. No cross-reactivity is observed to non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig or rabbit. -
Tested applications
Suitable for: Immunoelectrophoresis, IHC-Pmore details -
Immunogen
Full length protein corresponding to Mouse IgG. (Purified Mouse IgG, whole molecule).
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Conjugation
HRP polymer
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Proclin 300
Constituents: 1% BSA, 98% PBS
ab214879 is liquid with light red color. -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
ab214879 was affinity purified using solid phase Mouse IgG. Purity >95% by SDS-PAGE. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunoelectrophoresis |
Use at an assay dependent concentration.
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| IHC-P | (1) |
Use at an assay dependent concentration.
This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour. |
| Notes |
|---|
|
Immunoelectrophoresis
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Immunohistochemical analysis of Triple positive breast cancer tissue stained with Ki-67 and detected with ab214879.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab214879 has been referenced in 12 publications.
- Roper SJ & Coyle B Establishing an In Vitro 3D Spheroid Model to Study Medulloblastoma Drug Response and Tumor Dissemination. Curr Protoc 2:e357 (2022). PubMed: 35080813
- Ma N et al. Protective role of activating transcription factor 3 against neuronal damage in rats with cerebral ischemia. Brain Behav 12:e2522 (2022). PubMed: 35263513
- Nikolos F et al. Cell death-induced immunogenicity enhances chemoimmunotherapeutic response by converting immune-excluded into T-cell inflamed bladder tumors. Nat Commun 13:1487 (2022). PubMed: 35347124
- Kobayashi H et al. The Balance of Stromal BMP Signaling Mediated by GREM1 and ISLR Drives Colorectal Carcinogenesis. Gastroenterology 160:1224-1239.e30 (2021). PubMed: 33197448
- Roper SJ et al. 3D spheroid models of paediatric SHH medulloblastoma mimic tumour biology, drug response and metastatic dissemination. Sci Rep 11:4259 (2021). PubMed: 33608621