Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
Key features and details
- Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed
- Conjugation: IRDye® 680RD
- Host species: Goat
- Isotype: IgG
- Suitable for: In-Cell ELISA, WB
Related conjugates and formulations
Overview
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Product name
Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: In-Cell ELISA, WBmore details -
Minimal
cross-reactivity
Goat, Horse, Human, Rabbit, Rat more details -
Immunogen
Full length native protein (purified) corresponding to Mouse IgG.
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Conjugation
IRDye® 680RD
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS
Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer. -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Affinity chromatography purified using pooled mouse IgG covalently linked to agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| In-Cell ELISA |
1/200 - 1/800.
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| WB |
1/5000 - 1/25000.
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| Notes |
|---|
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In-Cell ELISA
1/200 - 1/800. |
|
WB
1/5000 - 1/25000. |
Images
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Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)All lanes : Anti-GAPDH antibody [6C5] - Loading Control (ab8245) at 1/10000 dilution (Anti-GAPDH antibody)
Lane 1 : Wild-type HAP1 cell lysate (20 µg)
Lane 2 : Brd4 knockout HAP1 cell lysate (20 µg)
Lane 3 : HeLa cell lysate (20 µg)
Secondary
All lanes : Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at 1/10000 dilution (Goat anti-Mouse IgG H&L (IRDye® 680RD))This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab128874 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773. Loading control to GAPDH ab8245 antibody binding was detected using the Goat anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Merged signal (red and green). Green Anti-Brd4 antibody [EPR5150(2)] ab128874 observed at 150 kDa using Goat anti-Rabbit IgG H&L (IRDye® 800CW)-ab216773 as secondary antibody. Red - Anti-GAPDH antibody loading control, ab8245, observed at 37 kDa,
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Spectrum - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab216776 has been referenced in 25 publications.
- David A et al. A common TMPRSS2 variant has a protective effect against severe COVID-19. Curr Res Transl Med 70:103333 (2022). PubMed: 35104687
- Ge Y et al. Alpha-Asaronol Alleviates Dysmyelination by Enhancing Glutamate Transport Through the Activation of PPARγ-GLT-1 Signaling in Hypoxia-Ischemia Neonatal Rats. Front Pharmacol 13:766744 (2022). PubMed: 35401225
- Reuschl AK et al. HIV-1 Vpr drives a tissue residency-like phenotype during selective infection of resting memory T cells. Cell Rep 39:110650 (2022). PubMed: 35417711
- Gao Z et al. Integrative Proteome and Ubiquitinome Analyses Reveal the Substrates of BTBD9 and Its Underlying Mechanism in Sleep Regulation. ACS Omega 7:11839-11852 (2022). PubMed: 35449961
- Yun D et al. G3BP2, a stress granule assembly factor, is dispensable for spermatogenesis in mice. PeerJ 10:e13532 (2022). PubMed: 35782098