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Goat Anti-Mouse IgG1 heavy chain (Alkaline Phosphatase) (ab97237)

Submit a review Q&A (2)References (4)

Key features and details

  • Goat Anti-Mouse IgG1 heavy chain (Alkaline Phosphatase)
  • Conjugation: Alkaline Phosphatase
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

Conjugates logo Related conjugates and formulations

APC APC/Cy7® Biotin FITC Unconjugated Unconjugated

Overview

  • Product name

    Goat Anti-Mouse IgG1 heavy chain (Alkaline Phosphatase)
    See all IgG1 secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%.

  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details

  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).

  • Conjugation notes

    Molar enzyme: antibody protein ratio is 1:1

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/100 - 1/1000.
ELISA
1/1000 - 1/10000.
WB
1/1000 - 1/10000. Use chemiluminescence.
Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/100 - 1/1000.
ELISA
1/1000 - 1/10000.
WB
1/1000 - 1/10000. Use chemiluminescence.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (4)

Publishing research using ab97237? Please let us know so that we can cite the reference in this datasheet.

ab97237 has been referenced in 4 publications.

  • Thomas S  et al. Development of a new fusion-enhanced oncolytic immunotherapy platform based on herpes simplex virus type 1. J Immunother Cancer 7:214 (2019). PubMed: 31399043
  • Lynch JM  et al. Binding of a glaucoma-associated myocilin variant to the aB-crystallin chaperone impedes protein clearance in trabecular meshwork cells. J Biol Chem 293:20137-20156 (2018). PubMed: 30389787
  • Lynch JM  et al. Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle. PLoS One 13:e0206801 (2018). PubMed: 30395621
  • Aguilar-Rodríguez J  et al. The Molecular Chaperone DnaK Is a Source of Mutational Robustness. Genome Biol Evol 8:2979-2991 (2016). PubMed: 27497316

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I have an antibody which works , but I am looking for an HRP tagged one :- Goat Anti-Mouse IgG1 heavy chain (Alkaline Phosphatase) (ab97237).

I was wondering if you had the exact same antibody with HRP tag.

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Answer

We do have the same antibody conjugated with HRP: Goat Anti-Mouse IgG1 heavy chain (HRP) (https://www.abcam.com/ab97240)

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Question

Thank you very much for your helpful information and time. I am looking for anti-mouse IgG2a and IgG1 secondary antibodies (either HRP- or AP- conjugated) to use for Isotyping of specific mouse serum IgGs against my desired antigen. I searched through your products but I could not find any product. I should say that the secondary antibodies I need must not cross-react with other mouse IgG subtypes. By the way, if you have any mouse IgG isotyping ELISA kit, I really appreciate it if you let me know. I am looking forward to hearing from you. Best Wishes,

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Answer

Thank you very much for your email. I have compiled here below a list of antibodies which I think could correspond to your needs. There are three different IgG1 and three different IgG2a antibodies which have been tested against other isotypes. Please have a look at them and do not hesitate to contact me again should you need any more information. The Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (HRP), pre-adsorbed (ab99603) has been tested by ELISA against a panel of purified Mouse Immunoglobulins including IgM, IgG1, IgG2a, IgG2b, IgG3, and IgA. This product is positive for IgG1 and has minimal reactivity with the other Mouse Immunoglobulins. https://www.abcam.com/Rat-monoclonal-SB77e-Mouse-IgG1-HL-HRP-preadsorbed-ab99603.html Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (AP) (ab97237) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Alkaline-Phosphatase-ab97237.html Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (HRP) (ab97240) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. This antibody may cross react with IgG1 from other species https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Alkaline-Phosphatase-ab97237.html Rat monoclonal [LO-MG2a-9] Secondary Antibody to Mouse IgG2a - heavy chain (HRP) (ab11571) ab11571 recognises murine gamma 2a immunoglobulin heavy chain, and does not cross-react with other murine immunoglobulin classes or subclasses. https://www.abcam.com/Rat-monoclonal-Mouse-IgG2a-heavy-chain-HRP-LO-MG2a-9-ab11571.html Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (HRP) (ab97245) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/Goat-Mouse-IgG2a-heavy-chain-HRP-ab97245.html Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (AP) (ab97242) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/Goat-Mouse-IgG2a-heavy-chain-Alkaline-Phosphatase-ab97242.html Unfortunately we do not have an immunoglobulin isotyping ELISA kit for the moment in our catalog. I have passed this feedback on and I hope we will have one in the future. I hope this information is helpful. Please do not hesitate to contact me again should you have any other question. I wish you a great weekend!

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