Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed (ab47827)
Key features and details
- Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, ELISA, WB, IHC-P, IHC-Fr, Electron Microscopy
Overview
-
Product name
Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed
See all IgG + IgM secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, Mouse IgG and Mouse IgM. -
Tested applications
Suitable for: ICC/IF, ELISA, WB, IHC-P, IHC-Fr, Electron Microscopymore details -
Minimal
cross-reactivity
Cow, Horse, Human more details -
Immunogen
Full length native proteins (purified) (Mouse)
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
IgG and protease free -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/500 - 1/2500.
|
|
| ELISA |
1/5000 - 1/40000.
|
|
| WB |
1/2000 - 1/10000.
|
|
| IHC-P |
1/500 - 1/2500.
|
|
| IHC-Fr |
1/500 - 1/2500.
|
|
| Electron Microscopy |
Use at an assay dependent dilution.
|
| Notes |
|---|
|
ICC/IF
1/500 - 1/2500. |
|
ELISA
1/5000 - 1/40000. |
|
WB
1/2000 - 1/10000. |
|
IHC-P
1/500 - 1/2500. |
|
IHC-Fr
1/500 - 1/2500. |
|
Electron Microscopy
Use at an assay dependent dilution. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (22)
ab47827 has been referenced in 22 publications.
- Zhu M et al. lncRNA LINC00284 promotes nucleus pulposus cell proliferation and ECM synthesis via regulation of the miR-205-3p/Wnt/ß-catenin axis. Mol Med Rep 25:N/A (2022). PubMed: 35322864
- Wu M et al. YTHDF2 interference suppresses the EMT of cervical cancer cells and enhances cisplatin chemosensitivity by regulating AXIN1. Drug Dev Res 83:1190-1200 (2022). PubMed: 35489084
- Hashimoto K et al. Clinicopathological assessment of cancer/testis antigens NY‑ESO‑1 and MAGE‑A4 in osteosarcoma. Eur J Histochem 66:N/A (2022). PubMed: 35736245
- Han XD et al. miRNA-124 regulates palmitic acid-induced epithelial-mesenchymal transition and cell migration in human retinal pigment epithelial cells by targeting LIN7C. Exp Ther Med 24:481 (2022). PubMed: 35761801
- Han H et al. miR-199b-3p contributes to acquired resistance to cetuximab in colorectal cancer by targeting CRIM1 via Wnt/ß-catenin signaling. Cancer Cell Int 22:42 (2022). PubMed: 35090460
