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Goat Anti-Mouse IgM mu chain (18nm Gold) preadsorbed (ab105293)

Submit a review Q&A (1)References (1)

Key features and details

  • Goat Anti-Mouse IgM mu chain (18nm Gold) preadsorbed
  • Conjugation: Gold 18nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: Electron Microscopy, WB, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 647 Alkaline Phosphatase APC Biotin DyLight® 488 DyLight® 594 FITC Gold 6nm HRP Unconjugated Unconjugated

Overview

  • Product name

    Goat Anti-Mouse IgM mu chain (18nm Gold) preadsorbed
    See all IgM secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    Recognizes rat IgM. Exhibits minimal crossreactivity to bovine, human and horse serum proteins.

  • Tested applications

    Suitable for: Electron Microscopy, WB, ELISAmore details

  • Minimal
    cross-reactivity


    Cow, Horse, Human more details

  • Conjugation

    Gold 18nm

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab105293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
1/10 - 1/20.
WB
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Notes
Electron Microscopy
1/10 - 1/20.
WB
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab105293? Please let us know so that we can cite the reference in this datasheet.

ab105293 has been referenced in 1 publication.

  • Barone A  et al. Sialyl-lactotetra, a novel cell surface marker of undifferentiated human pluripotent stem cells. J Biol Chem 289:18846-59 (2014). Electron Microscopy ; Mouse . PubMed: 24841197

Customer reviews and Q&As

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Submit a review Submit a question

Question

Can you tell me the concentration (mg/ml) for these 2 products, or the recommended dilution for Electron Microscopy?

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Answer

Thank you for your enquiry.

We do not know the antibody concentration in gold complexes because we separate monomers from multimers on density gradients. We do not have any reliable way to measure the antibody concentration on the monomers because the preparation is stabilized with albumin before and after centrifugation.

Optimal dilutions should be determined by researcher, but a recommended range for these two antibodies in EM is 1:10-1:20.

I hope this information will still be helpful.

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